BMRB Entry 36162

Title:
Solution structure of the SBDalpha domain of yeast Ssa1
Deposition date:
2018-02-01
Original release date:
2018-10-12
Authors:
Gong, W.; Hu, W.; Perrett, S.
Citation:

Citation: Gong, W.; Hu, W.; Xu, L.; Wu, H.; Wu, S.; Zhang, H.; Wang, J.; Jones, G.; Perrett, S.. "The C-terminal GGAP motif of Hsp70 mediates substrate recognition and stress response in yeast"  J. Biol. Chem. 293, 17663-17675 (2018).
PubMed: 30228181

Assembly members:

Assembly members:
entity_1, polymer, 101 residues, 10788.742 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 559292   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts405
15N chemical shifts107
1H chemical shifts616

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 101 residues - 10788.742 Da.

1   SERLYSGLUGLUASPGLULYSGLUSERGLN
2   ARGILEALASERLYSASNGLNLEUGLUSER
3   ILEALATYRSERLEULYSASNTHRILESER
4   GLUALAGLYASPLYSLEUGLUGLNALAASP
5   LYSASPTHRVALTHRLYSLYSALAGLUGLU
6   THRILESERTRPLEUASPSERASNTHRTHR
7   ALASERLYSGLUGLUPHEASPASPLYSLEU
8   LYSGLULEUGLNASPILEALAASNPROILE
9   METSERLYSLEUTYRGLNALAGLYGLYALA
10   PROGLYGLYALAALAGLYGLYALAPROGLY
11   GLY

Samples:

sample_1: Ssa1 SBDalpha, [U-13C; U-15N], 1.0 mM; H2O 90%; D2O, [U-99% 2H], 10%

sample_conditions_1: ionic strength: 90 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks