BMRB Entry 36103

Title:
Pholiota squarrosa lectin trimer
Deposition date:
2017-07-12
Original release date:
2018-06-07
Authors:
Yamasaki, K.
Citation:

Citation: Yamasaki, Kazuhiko; Yamasaki, Tomoko; Tateno, Hiroaki. "The trimeric solution structure and fucose-binding mechanism of the core fucosylation-specific lectin PhoSL."  Sci. Rep. 8, 7740-7740 (2018).
PubMed: 29773815

Assembly members:

Assembly members:
entity_1, polymer, 40 residues, 4440.958 Da.

Natural source:

Natural source:   Common Name: Pholiota squarrosa   Taxonomy ID: 75321   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Pholiota squarrosa

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: APVPVTKLVCDGDTYKCTAY LDYGDGKWVAQWDTAVFHTT

Data sets:
Data typeCount
1H chemical shifts270

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1, 11
2entity_1, 21
3entity_1, 31

Entities:

Entity 1, entity_1, 1 40 residues - 4440.958 Da.

1   ALAPROVALPROVALTHRLYSLEUVALCYS
2   ASPGLYASPTHRTYRLYSCYSTHRALATYR
3   LEUASPTYRGLYASPGLYLYSTRPVALALA
4   GLNTRPASPTHRALAVALPHEHISTHRTHR

Samples:

sample_1: Pholiota squarrosa lectin (PhoSL) 0.3 mM; TRIS 20 mM; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1isotropicsample_conditions_1
2D TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

Felix v2007, Accelrys Software Inc. - data analysis

NMR spectrometers:

  • Bruker AvanceIII HD 900 MHz