BMRB Entry 36079

Title:
Beta-Ginkgotides: Hyperdisulfide-constrained peptides from Ginkgo biloba
Deposition date:
2017-04-27
Original release date:
2018-01-17
Authors:
Wong, K.; Tan, W.; Xiao, T.; Tam, J.
Citation:

Citation: Wong, K.; Tan, W.; Xiao, T.; Tam, J.. "Beta-Ginkgotides: Hyperdisulfide-constrained peptides from Ginkgo biloba"  Sci. Rep. 7, 6140-6140 (2017).
PubMed: 28733600

Assembly members:

Assembly members:
entity_1, polymer, 20 residues, 2384.799 Da.

Natural source:

Natural source:   Common Name: Ginkgo biloba   Taxonomy ID: 3311   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Ginkgo biloba

Experimental source:

Experimental source:   Production method: na

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: YETGCKRCCYLDEYGCIRCC

Data sets:
Data typeCount
1H chemical shifts102

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 20 residues - 2384.799 Da.

1   TYRGLUTHRGLYCYSLYSARGCYSCYSTYR
2   LEUASPGLUTYRGLYCYSILEARGCYSCYS

Samples:

sample_1: beta-ginkgotide, beta-gB1 2 mM; TFA 0.1%; D2O, [U-2H], 5%; H2O 95%

sample_conditions_1: ionic strength: 0 mM; pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker AvanceII 600 MHz