BMRB Entry 34482

Title:
Constitutive decay element CDE2 from human 3'UTR
Deposition date:
2020-01-23
Original release date:
2020-05-25
Authors:
Schwalbe, H.; Binas, O.
Citation:

Citation: Binas, O.; Tants, J.; Peter, S.; Janowski, R.; Davydova, E.; Braun, J.; Niessing, D.; Schwalbe, H.; Weigand, J.; Schlundt, A.. "Structural basis for the recognition of transiently structured AU-rich elements by Roquin"  Nucleic Acids Res. 48, 7385-7403 (2020).
PubMed: 32491174

Assembly members:

Assembly members:
entity_1, polymer, 21 residues, 6690.004 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGUGCCUAAUAUUUAGGCAC C

Data sets:
Data typeCount
13C chemical shifts94
1H chemical shifts159

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 21 residues - 6690.004 Da.

1   GGUGCCUAAU
2   AUUUAGGCAC
3   C

Samples:

sample_1: CDE2 500 uM; KCl 50 mM; potassium phosphate buffer 25 mM

sample_2: CDE2, [U-99% 13C; U-99% 15N], 1300 uM; KCl 50 mM; potassium phosphate buffer 25 mM

sample_conditions_1: ionic strength: 75 mM; pH: 6.2; pressure: 1 bar; temperature: 298 K

sample_conditions_2: ionic strength: 75 mM; pH: 6.2; pressure: 1 bar; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_2
gamma_HCNsample_2isotropicsample_conditions_2
fwHCC-TOCSY-CCH-E.COSYsample_2isotropicsample_conditions_2
3D HCPsample_2isotropicsample_conditions_2
2D PFIDSsample_2isotropicsample_conditions_2
3D HCP-TOCSYsample_2isotropicsample_conditions_2
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HCNsample_2isotropicsample_conditions_2

Software:

CNS v1.1, Brunger A. T. et.al. - structure calculation

X-PLOR NIH v2.52, Schwieters, Kuszewski, Tjandra and Clore - refinement

Sparky v3, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE III 700 MHz
  • Bruker AVANCE III 950 MHz