BMRB Entry 34400

Title:
RW16 peptide
Deposition date:
2019-05-16
Original release date:
2019-10-21
Authors:
Jobin, M.; Grelard, A.; Alves, I.; Mackereth, C.
Citation:

Citation: Jobin, M.; Vamparys, L.; Deniau, R.; Grelard, A.; Mackereth, C.; Fuchs, P.; Alves, I.. "Biophysical Insight on the Membrane Insertion of an Arginine-Rich Cell-Penetrating Peptide."  Int. J. Mol. Sci. 20, E4441-E4441 (2019).
PubMed: 31505894

Assembly members:

Assembly members:
entity_1, polymer, 18 residues, 3032.639 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: synthetic construct

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XXRRWRRWWRRWWRRWRR

Data sets:
Data typeCount
13C chemical shifts30
1H chemical shifts146

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 18 residues - 3032.639 Da.

1   BTNKFBARGARGTRPARGARGTRPTRPARG
2   ARGTRPTRPARGARGTRPARGARG

Samples:

sample_1: RW16 peptide 1 mM; DPC, [U-100% 2H], 60 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

TopSpin v3.2, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Sparky v3.113, Goddard - data analysis

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

ARIA v1.2, Linge, O'Donoghue and Nilges - structure calculation

NMR spectrometers:

  • Bruker AVANCE III 800 MHz