BMRB Entry 34298

Title:
Solution structure of Melampsora larici-populina MlpP4.1
Deposition date:
2018-07-09
Original release date:
2018-08-16
Authors:
Tsan, P.
Citation:

Citation: de Guillen, K.; Lorrain, C.; Tsan, P.; Barthe, P.; Petre, B.; Saveleva, N.; Rouhier, N.; Duplessis, S.; Padilla, A.; Hecker, A.. "Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds."  Sci. Rep. 9, 18084-18084 (2019).
PubMed: 31792250

Assembly members:

Assembly members:
entity_1, polymer, 75 residues, 7978.616 Da.

Natural source:

Natural source:   Common Name: Melampsora larici-populina   Taxonomy ID: 747676   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Melampsora larici-populina

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts115
15N chemical shifts67
1H chemical shifts318

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 75 residues - 7978.616 Da.

1   METCYSGLUPHEILEGLUASPSERGLUASP
2   ILEGLNGLYLEULYSSERLEUARGLYSSER
3   HISTHRSERLEUGLUASPASPASPASPGLY
4   SERARGGLYGLYASPCYSGLUGLYCYSSER
5   GLYTHRALACYSSERSERASPALAGLNCYS
6   ARGALAARGGLYCYSASPGLYCYSSERTHR
7   SERGLYVALCYSVALLEUSERSERLEUHIS
8   HISHISHISHISHIS

Samples:

sample_1: entity_1, [U-15N], 1.8 mM; potassium phosphate 50 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis

ARIA, Linge, O'Donoghue and Nilges - structure calculation

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Bruker AvanceIII 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks