BMRB Entry 34284

Title:
NMR structure of aurein 2.5 in SDS micelles
Deposition date:
2018-06-13
Original release date:
2018-07-13
Authors:
Manzo, G.; Mason, J.
Citation:

Citation: Manzo, Giorgia; Ferguson, Philip; Hind, Charlotte; Clifford, Melanie; Gustilo, V Benjamin; Ali, Hind; Bansal, Sukhvinder; Bui, Tam; Drake, Alex; Atkinson, R Andrew; Sutton, J Mark; Lorenz, Christian; Phoenix, David; Mason, A James. "Temporin L and aurein 2.5 have identical conformations but subtly distinct membrane and antibacterial activities"  Sci. Rep. 9, 10934-10934 (2019).
PubMed: 31358802

Assembly members:

Assembly members:
entity_1, polymer, 16 residues, 1651.987 Da.

Natural source:

Natural source:   Common Name: green and golden bell frog   Taxonomy ID: 8371   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Litoria aurea

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GLFDIVKKVVGAFGSL

Data sets:
Data typeCount
1H chemical shifts114

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 16 residues - 1651.987 Da.

1   GLYLEUPHEASPILEVALLYSLYSVALVAL
2   GLYALAPHEGLYSERLEU

Samples:

sample_1: Aurein 2.5 2 mM; deuterated sodium dodecyl sulphate, [U-98% 2H], 100 mM; 3-(Trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt, [U-2H], 0.05 % w/w

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

DYNAMO, Delaglio and Kuszewski - refinement, structure calculation

CARA, Keller and Wuthrich - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Bruker AvanceII 700 MHz