BMRB Entry 34212

Title:
NMR structure of purotoxin-6
Deposition date:
2017-12-04
Original release date:
2018-12-14
Authors:
Nadezhdin, K.; Oparin, P.; Vassilevski, A.; Arseniev, A.; Grishin, E.
Citation:

Citation: Nadezhdin, K.. "NMR structure of purotoxin-6"  .

Assembly members:

Assembly members:
entity_1, polymer, 31 residues, 3327.023 Da.

Natural source:

Natural source:   Common Name: Thomisus onustus   Taxonomy ID: 1872008   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Thomisus onustus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'   Vector: pET-32b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GYCATKGIKCNDIHCCSGLK CDSKRKVCVKG

Data sets:
Data typeCount
13C chemical shifts78
1H chemical shifts213

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 31 residues - 3327.023 Da.

1   GLYTYRCYSALATHRLYSGLYILELYSCYS
2   ASNASPILEHISCYSCYSSERGLYLEULYS
3   CYSASPSERLYSARGLYSVALCYSVALLYS
4   GLY

Samples:

sample_1: purotoxin-6 0.6 mM

sample_2: purotoxin-6 0.6 mM

sample_conditions_1: ionic strength: 0 mM; pH: 3.0; pressure: 1 atm; temperature: 303 K

sample_conditions_2: ionic strength: 0 mM; pH: 3.0 pD; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1isotropicsample_conditions_1
2D TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D NOESYsample_2isotropicsample_conditions_2

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz