BMRB Entry 34014

Title:
NMR structure of Chicken AvBD7 defensin
Deposition date:
2016-06-22
Original release date:
2016-09-02
Authors:
Landon, C.; Loth, K.; Meudal, H.
Citation:

Citation: Bailleul, G.; Kravtzoff, A.; Joulin-Giet, A.; Lecaille, F.; Labas, V.; Meudal, H.; Loth, K.; Teixeira-Gomes, A.; Gilbert, F.; Coquet, L.; Jouenne, T.; Bromme, D.; Schouler, C.; Landon, C.; Lalmanach, G.; Lalmanach, A.. "The Unusual Resistance of Avian Defensin AvBD7 to Proteolytic Enzymes Preserves Its Antibacterial Activity."  Plos One 11, e0161573-e0161573 (2016).
PubMed: 27561012

Assembly members:

Assembly members:
entity_1, polymer, 47 residues, 5366.268 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XPFIPRPIDTCRLRNGICFP GICRRPYYWIGTCNNGIGSC CARGWRS

Data sets:
Data typeCount
13C chemical shifts136
15N chemical shifts43
1H chemical shifts323

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 47 residues - 5366.268 Da.

1   PCAPROPHEILEPROARGPROILEASPTHR
2   CYSARGLEUARGASNGLYILECYSPHEPRO
3   GLYILECYSARGARGPROTYRTYRTRPILE
4   GLYTHRCYSASNASNGLYILEGLYSERCYS
5   CYSALAARGGLYTRPARGSER

Samples:

sample_1: Chicken AvBD7 0.23 mM

sample_conditions_1: ionic strength: 0 M; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - structure calculation

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CcpNMR, CCPN - chemical shift assignment, peak picking

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker AvanceIII 950 MHz
  • Bruker AvanceIII 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks