BMRB Entry 30723

Title:
Cap3G-TAR-F1
Deposition date:
2020-02-14
Original release date:
2020-04-27
Authors:
Summers, M.; Brown, J.
Citation:

Citation: Brown, Joshua; Kharytonchyk, Siarhei; Chaudry, Issac; Iyer, Aishwarya; Carter, Hannah; Becker, Ghazal; Desai, Yash; Glang, Lindsay; Choi, Seung; Singh, Karndeep; Lopresti, Michael; Orellana, Matthew; Rodriguez, Tatiana; Oboh, Ubiomo; Hijji, Jana; Ghinger, Frances Grace; Stewart, Kailan; Francis, Dillion; Edwards, Bryce; Chen, Patrick; Case, David; Telesnitsky, Alice; Summers, Michael. "Structural basis for transcriptional start site control of HIV-1 RNA fate"  Science 368, 413-417 (2020).
PubMed: 32327595

Assembly members:

Assembly members:
entity_1, polymer, 34 residues, 11041.558 Da.

Natural source:

Natural source:   Common Name: HIV-1   Taxonomy ID: 11676   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus HIV-1

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XGGGUCUCUCUUGUCGAGAG GCAAGGAACCCACU

Data sets:
Data typeCount
1H chemical shifts160

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 34 residues - 11041.558 Da.

1   8NKGGGUCUCUC
2   UUGUCGAGAG
3   GCAAGGAACC
4   CACU

Samples:

sample_1: Cap3G-TAR-F1, Fully protonated, 150 uM; KH2PO4, Deuterated, 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.4 pD; pressure: 1 atm; temperature: 308 K

sample_conditions_2: ionic strength: 10 mM; pH: 7.4; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_2

Software:

Amber, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, and Kollman - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMRFx, Johnson, One Moon Scientific - processing

NMR spectrometers:

  • Bruker AVANCE 600 MHz