BMRB Entry 30643

Title:
Backbone-modified variant of zinc finger 2 from the transcription factor Sp1 DNA binding domain: Orn in the metal-binding turn
Deposition date:
2019-07-19
Original release date:
2020-06-19
Authors:
Rao, S.; Horne, W.
Citation:

Citation: Rao, S.; Horne, W.. "Proteomimetic Zinc Finger Domains with Modified Metal-binding beta-Turns"  Pept. Sci. (Hoboken) 112, e24177-e24177 (2020).
PubMed: 33733039

Assembly members:

Assembly members:
entity_1, polymer, 31 residues, 3697.285 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RPFXCTWXCGKRFTRSDELQ RHKRTHTGEKX

Data sets:
Data typeCount
1H chemical shifts206

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2ZINC ION2

Entities:

Entity 1, entity_1 31 residues - 3697.285 Da.

1   ARGPROPHENLECYSTHRTRPORNCYSGLY
2   LYSARGPHETHRARGSERASPGLULEUGLN
3   ARGHISLYSARGTHRHISTHRGLYGLULYS
4   NH2

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: entity_1 1.2 mM; TRIS, [U-2H], 10 mM; DSS 0.05 mM; zinc chloride 1.8 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6 pH*; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - processing

Sparky, Goddard - data analysis

ARIA, Linge, O'Donoghue and Nilges - refinement, structure calculation

NMR spectrometers:

  • Bruker Avance 700 MHz