BMRB Entry 30637

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30637
MolProbity Validation Chart

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Title:
Structural Basis for Client Recognition and Activity of Hsp40 Chaperones
Deposition date:
2019-07-10
Original release date:
2019-09-11
Authors:
Jiang, Y.; Rossi, P.; Kalodimos, C.
Citation:

Citation: Jiang, Y.; Rossi, P.; Kalodimos, C.. "Structural basis for client recognition and activity of Hsp40 chaperones"  Science 365, 1313-1319 (2019).
PubMed: 31604242

Assembly members:

Assembly members:
entity_1, polymer, 161 residues, 16625.180 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 83333   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AALVAHVTSGSGGSGGSGGS GRDLRAELPLTLEEAFHGGE RVVEVAGRRVSVRIPPGVRE GSVIRVPGMGGQGNPPGDLL LVVRLLPHPVFRLEGQDLYA TLDVPAPIAVVGGKVRAMTL EGPVEVAVPPRTQAGRKLRL KGKGFPGPAGRGDLYLEVRI T

Data sets:
Data typeCount
13C chemical shifts311
15N chemical shifts132
1H chemical shifts426

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 161 residues - 16625.180 Da.

1   ALAALALEUVALALAHISVALTHRSERGLY
2   SERGLYGLYSERGLYGLYSERGLYGLYSER
3   GLYARGASPLEUARGALAGLULEUPROLEU
4   THRLEUGLUGLUALAPHEHISGLYGLYGLU
5   ARGVALVALGLUVALALAGLYARGARGVAL
6   SERVALARGILEPROPROGLYVALARGGLU
7   GLYSERVALILEARGVALPROGLYMETGLY
8   GLYGLNGLYASNPROPROGLYASPLEULEU
9   LEUVALVALARGLEULEUPROHISPROVAL
10   PHEARGLEUGLUGLYGLNASPLEUTYRALA
11   THRLEUASPVALPROALAPROILEALAVAL
12   VALGLYGLYLYSVALARGALAMETTHRLEU
13   GLUGLYPROVALGLUVALALAVALPROPRO
14   ARGTHRGLNALAGLYARGLYSLEUARGLEU
15   LYSGLYLYSGLYPHEPROGLYPROALAGLY
16   ARGGLYASPLEUTYRLEUGLUVALARGILE
17   THR

Samples:

sample_1: C-CBD1-CBD2, [ILVMAT-sel-1H-13C_methyls; U-15N; U-2H], 1 mM; potassium chloride 75 mM; potassium phosphate 20 mM; sodium azide 0.04%

sample_2: C-CBD1-CBD2, [U-100% 13C; U-100% 15N], 1 mM; potassium chloride 75 mM; potassium phosphate 20 mM; sodium azide 0.04%

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HCH-SFNOESYsample_1isotropicsample_conditions_1
3D CNH-SFNOESYsample_1isotropicsample_conditions_1
3D NCH-SFNOESYsample_1isotropicsample_conditions_1
3D CCH-SFNOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

Sparky, Goddard - peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

TopSpin v4.0, Bruker Biospin - collection

PSVS, Bhattacharya and Montelione - geometry optimization

NMR spectrometers:

  • Bruker AVANCE NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks