BMRB Entry 30631

Title:
Solution structure of OlvA(BC)
Deposition date:
2019-07-09
Original release date:
2019-10-14
Authors:
Acedo, J.; van der Donk, W.
Citation:

Citation: Acedo, Jeella; Bothwell, Ian; An, Linna; Trouth, Abby; Frazier, Clara; van der Donk, Wilfred. "O-Methyltransferase-Mediated Incorporation of a beta-Amino Acid in Lanthipeptides"  J. Am. Chem. Soc. 141, 16790-16801 (2019).
PubMed: 31568727

Assembly members:

Assembly members:
entity_1, polymer, 19 residues, 1616.841 Da.

Natural source:

Natural source:   Common Name: Streptomyces olivaceus   Taxonomy ID: 47716   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces olivaceus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ACGXGDGCAKXCAASCAAS

Data sets:
Data typeCount
1H chemical shifts84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 19 residues - 1616.841 Da.

1   ALACYSGLYDBBGLYASPGLYCYSALALYS
2   DBBCYSALAALASERCYSALAALASER

Samples:

sample_1: OlvA(BC) 2.5 mM; sodium phosphate 30 mM; DSS 0.001%

sample_2: OlvA(BC) 2.5 mM; sodium phosphate 30 mM; DSS 0.001%

sample_conditions_1: ionic strength: 30 mM; pH: 5.8; pressure: 760 mmHg; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 750 MHz