BMRB Entry 30623

Title:
Solution structure of the C-terminal zinc finger of the C. elegans protein MEX-5
Deposition date:
2019-07-01
Original release date:
2020-04-27
Authors:
Massi, F.; Tavella, D.
Citation:

Citation: Tavella, Davide; Ertekin, Asli; Schaal, Hila; Ryder, Sean; Massi, Francesca. "A Disorder-to-Order Transition Mediates RNA Binding of the Caenorhabditis elegans Protein MEX-5"  Biophys. J. 118, 2001-2014 (2020).
PubMed: 32294479

Assembly members:

Assembly members:
entity_1, polymer, 35 residues, 4031.641 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: C. elegans   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: NNKYKTKLCKNFARGGTGFC PYGLRCEFVHPTDKE

Data typeCount
13C chemical shifts114
15N chemical shifts32
1H chemical shifts222

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 35 residues - 4031.641 Da.

1   ASNASNLYSTYRLYSTHRLYSLEUCYSLYS
2   ASNPHEALAARGGLYGLYTHRGLYPHECYS
3   PROTYRGLYLEUARGCYSGLUPHEVALHIS
4   PROTHRASPLYSGLU

Entity 2, entity_2 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: MEX-5, [U-13C; U-15N], 300 nM

sample_conditions_1: ionic strength: 100 mM; pH: 6.2; pressure: 1 atm; temperature: 294 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

Sparky, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks