BMRB Entry 30588

Title:
Syn-safencin 56
Deposition date:
2019-03-11
Original release date:
2020-05-11
Authors:
Fields, F.; Lee, S.
Citation:

Citation: Fields, Francisco; Manzo, Giorgia; Hind, Charlotte; Janardhanan, Jeshina; Foik, Ilona; Carmo Silva, Phoebe Do; Balsara, Rashna; Clifford, Melanie; Vu, Henry; Ross, Jessica; Kalwajtys, Veronica; Gonzalez, Alejandro; Bui, Tam; Ploplis, Victoria; Castellino, Francis; Siryaporn, Albert; Chang, Mayland; Sutton, J Mark; Mason, A James; Lee, Shaun. "Synthetic Antimicrobial Peptide Tuning Permits Membrane Disruption and Interpeptide Synergy"  ACS Pharmacol. Transl. Sci. 3, 418-424 (2020).
PubMed: 32566907

Assembly members:

Assembly members:
entity_1, polymer, 25 residues, 3061.819 Da.

Natural source:

Natural source:   Common Name: Bacillus safensis   Taxonomy ID: 561879   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus safensis

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AGKEKIRKKLKNEIKKKWRK AVIAW

Data sets:
Data typeCount
1H chemical shifts70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 25 residues - 3061.819 Da.

1   ALAGLYLYSGLULYSILEARGLYSLYSLEU
2   LYSASNGLUILELYSLYSLYSTRPARGLYS
3   ALAVALILEALATRP

Samples:

sample_1: Syn-safencin 56 500 uM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, peak picking, refinement, structure calculation

NMR spectrometers:

  • Bruker AVANCE 500 MHz