BMRB Entry 30509

Title:
HS02 - Intragenic antimicrobial peptides derived from the protein unconventional myosin 1h
Deposition date:
2018-08-30
Original release date:
2019-08-06
Authors:
Santos, M.; Brand, G.; Oliveira, A.
Citation:

Citation: Brand, Guilherme; Ramada, Marcelo; Manickchand, Julia; Correa, Rafael; Ribeiro, Dalila; Santos, Michele; Vasconcelos, Andreanne; Abrao, Fernando; Prates, Maura; Murad, Andre; Cardozo Fh, Jose; Leite, Jose Roberto; Magalhaes, Kelly; Oliveira, Aline; Bloch, Carlos. "Intragenic antimicrobial peptides (IAPs) from human proteins with potent antimicrobial and anti-inflammatory activity"  PLoS One 14, e0220656-e0220656 (2019).
PubMed: 31386688

Assembly members:

Assembly members:
entity_1, polymer, 16 residues, 1967.469 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KWAVRIIRKFIKGFIS

Data sets:
Data typeCount
1H chemical shifts120

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 16 residues - 1967.469 Da.

1   LYSTRPALAVALARGILEILEARGLYSPHE
2   ILELYSGLYPHEILESER

Samples:

sample_1: peptide 2 ± 0.2 uM; Dodecylphosphorylcholine-d38, D-98%, 50 ± 0.2 uM; PBS 10 ± 0.2 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

ARIA v2.3.1, Brunger, Adams, Clore, Gros, Nilges and Read, Linge, O'Donoghue and Nilges - refinement, structure calculation

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

CcpNMR v2.4, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance III HD 600 MHz