BMRB Entry 30505

Title:
Solution NMR Structure of a Class I Hydrophobin from Wallemia ichthyophaga
Deposition date:
2018-08-01
Original release date:
2019-08-02
Authors:
Kenward, C.; Langelaan, D.
Citation:

Citation: Kenward, C.; Langelaan, D.. "Solution NMR Structure of a Class I Hydrophobin from Wallemia ichthyophaga"  .

Assembly members:

Assembly members:
entity_1, polymer, 90 residues, 9444.642 Da.

Natural source:

Natural source:   Common Name: Wallemia ichthyophaga   Taxonomy ID: 1299270   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Wallemia ichthyophaga

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli DH1

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts365
15N chemical shifts88
1H chemical shifts560

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 90 residues - 9444.642 Da.

1   GLYSERGLYTRPGLUSERLYSTHRGLYSER
2   CYSASNTHRGLYLYSLEUALACYSCYSASP
3   THRASNLYSLYSVALGLNLYSSERTHRGLY
4   GLUGLUSERGLYLEULEUHISTHRGLYASP
5   VALLEUASPGLNVALALAILEGLNCYSTHR
6   GLNILEPROLEULEUILEGLYILEALAILE
7   GLUASPGLUCYSLYSASNTHRPROTHRCYS
8   CYSGLUASPVALGLUASPASPGLYLEUVAL
9   GLYILEASNCYSTHRPROILEPROLEUILE

Samples:

sample_1: WI1, [U-13C; U-15N], 250 uM; MES 20 mM; sodium chloride 50 mM

sample_2: WI1, [U-13C; U-15N], 100 uM; MES 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 303.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

ARIA v2.3.1, Linge, O'Donoghue and Nilges - refinement, structure calculation

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker AvanceIII 800 MHz
  • Bruker AvanceIII 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks