BMRB Entry 30479

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30479
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Title:
NMR solution structure of Acan1 from the Ancylostoma caninum hookworm
Deposition date:
2018-06-11
Original release date:
2019-06-13
Authors:
Smallwood, T.; Rosengren, K.; Clark, R.
Citation:

Citation: Smallwood, T.; Rosengren, K.; Clak, R.. "NMR solution structure of Acan1 from the Ancylostoma caninum hookworm"  .

Assembly members:

Assembly members:
entity_1, polymer, 41 residues, 4641.169 Da.

Natural source:

Natural source:   Common Name: dog hookworm   Taxonomy ID: 29170   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ancylostoma caninum

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: EHCADEKNFDCRRSLRNGDC DNDDKLLEMGYYCPVTCGFC X

Data sets:
Data typeCount
13C chemical shifts76
15N chemical shifts34
1H chemical shifts230

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 41 residues - 4641.169 Da.

1   GLUHISCYSALAASPGLULYSASNPHEASP
2   CYSARGARGSERLEUARGASNGLYASPCYS
3   ASPASNASPASPLYSLEULEUGLUMETGLY
4   TYRTYRCYSPROVALTHRCYSGLYPHECYS
5   NH2

Samples:

sample_1: Acan1 1 mg/mL

sample_conditions_1: pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

TOPSPIN, Bruker Biospin - collection, processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks