BMRB Entry 30413

Title:
JzTx-V toxin peptide, wild-type
Deposition date:
2018-02-22
Original release date:
2018-05-10
Authors:
Jordan, J.
Citation:

Citation: Moyer, B.; Murray, J.; Ligutti, J.; Andrews, K.; Favreau, P.; Jordan, J.; Lee, J.; Liu, D.; Long, J.; Sham, K.; Shi, L.; Stocklin, R.; Wu, B.; Yin, R.; Yu, V.; Zou, A.; Biswas, K.; Miranda, L.. "Pharmacological characterization of potent and selective NaV1.7 inhibitors engineered from Chilobrachys jingzhao tarantula venom peptide JzTx-V."  PLoS ONE 13, e0196791-e0196791 (2018).
PubMed: 29723257

Assembly members:

Assembly members:
entity_1, polymer, 30 residues, 3758.600 Da.

Natural source:

Natural source:   Common Name: Chinese earth tiger tarantula   Taxonomy ID: 278060   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Chilobrachys guangxiensis

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RYCQKWXWTCDSKRACCEGL RCKLWCRKII

Data sets:
Data typeCount
1H chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 30 residues - 3758.600 Da.

1   ARGTYRCYSGLNLYSTRPNLETRPTHRCYS
2   ASPSERLYSARGALACYSCYSGLUGLYLEU
3   ARGCYSLYSLEUTRPCYSARGLYSILEILE

Samples:

sample_1: JzTx-V toxin peptide, wild type 5 mg/mL; Sodium Phosphate 20 mM; Sodium Chloride 50 mM

sample_conditions_1: ionic strength: 70 mM; pH: 7; pressure: 760 Torr; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D HMBCsample_1isotropicsample_conditions_1

Software:

CYANA v3, Guntert, Mumenthaler and Wuthrich - structure calculation

SPARKY, Goddard - chemical shift assignment, peak picking

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Bruker AvanceIII 500 MHz
  • Bruker AvanceIII 600 MHz