BMRB Entry 30404

Title:
NMR structure of the Rous sarcoma virus matrix protein (M-domain) in the presence of myo-inositol hexakisphosphate
Deposition date:
2018-02-11
Original release date:
2018-10-15
Authors:
Vlach, J.; Saad, J.
Citation:

Citation: Vlach, Jiri; Eastep, Gunnar; Ghanam, Ruba; Watanabe, Susan; Carter, Carol; Saad, Jamil. "Structural basis for targeting avian sarcoma virus Gag polyprotein to the plasma membrane for virus assembly"  J. Biol. Chem. 293, 18828-18840 (2018).
PubMed: 30309983

Assembly members:

Assembly members:
entity_1, polymer, 87 residues, 9209.771 Da.

Natural source:

Natural source:   Common Name: RSV-PrC   Taxonomy ID: 11888   Superkingdom: Viruses   Kingdom: not available   Genus/species: Alpharetrovirus Rous sarcoma virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts379
15N chemical shifts87
1H chemical shifts615

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 87 residues - 9209.771 Da.

1   SERGLUALAVALILELYSVALILESERSER
2   ALACYSLYSTHRTYRCYSGLYLYSTHRSER
3   PROSERLYSLYSGLUILEGLYALAMETLEU
4   SERLEULEUGLNLYSGLUGLYLEULEUMET
5   SERPROSERASPLEUTYRSERPROGLYSER
6   TRPASPPROILETHRALAALALEUSERGLN
7   ARGALAMETILELEUGLYLYSSERGLYGLU
8   LEULYSTHRTRPGLYLEUVALLEUGLYALA
9   LEULYSALAALAARGGLUGLU

Samples:

sample_1: Matrix protein, [U-95% 15N], 0.5 mM; sodium phosphate 50 mM; tcep 2 mM; IP6 2 mM

sample_2: Matrix protein, [U-95% 13C; U-95% 15N], 0.5 mM; sodium phosphate 50 mM; tcep 2 mM; IP6 2 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

unio, Herrmann - structure calculation

NMR spectrometers:

  • Bruker AvanceII 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks