BMRB Entry 30397

Title:
Zika virus capsid protein
Deposition date:
2018-01-11
Original release date:
2019-01-11
Authors:
Morando, M.; Barbosa, G.; Cruz-Oliveira, C.; Da Poian, A.; Almeida, F.
Citation:

Citation: Morando, M.; Barbosa, G.; Cruz-Oliveira, C.; Da Poian, A.; Almeida, F.. "Dynamics of Zika Virus Capsid Protein in Solution: The Properties and Exposure of the Hydrophobic Cleft Are Controlled by the alpha-Helix 1 Sequence"  Biochemistry 58, 2488-2498 (2019).
PubMed: 31034208

Assembly members:

Assembly members:
entity_1, polymer, 104 residues, 11676.451 Da.

Natural source:

Natural source:   Common Name: ZIKV   Taxonomy ID: 64320   Superkingdom: Viruses   Kingdom: not available   Genus/species: Flavivirus ZIKV

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3a

Data sets:
Data typeCount
13C chemical shifts459
15N chemical shifts98
1H chemical shifts750

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1, chain 11
2entity_1, chain 21

Entities:

Entity 1, entity_1, chain 1 104 residues - 11676.451 Da.

1   METLYSASNPROLYSLYSLYSSERGLYGLY
2   PHEARGILEVALASNMETLEULYSARGGLY
3   VALALAARGVALSERPROPHEGLYGLYLEU
4   LYSARGLEUPROALAGLYLEULEULEUGLY
5   HISGLYPROILEARGMETVALLEUALAILE
6   LEUALAPHELEUARGPHETHRALAILELYS
7   PROSERLEUGLYLEUILEASNARGTRPGLY
8   SERVALGLYLYSLYSGLUALAMETGLUILE
9   ILELYSLYSPHELYSLYSASPLEUALAALA
10   METLEUARGILEILEASNALAARGLYSGLU
11   LYSLYSARGARG

Samples:

sample_1: ZIKVC, [U-99% 13C; U-99% 15N], 300 ± 0.1 uM

sample_conditions_1: ionic strength: 0.2323 M; pH: 6.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CNS v1.21, Brunger A. T. et.al. - refinement

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

CcpNMR v2.4.2, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AvanceIII 600 MHz
  • Bruker AvanceIIIHD 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks