BMRB Entry 30338

Title:
PawL-Derived Peptide PLP-4
Deposition date:
2017-09-05
Original release date:
2018-03-01
Authors:
Fisher, M.; Mylne, J.; Howard, M.
Citation:

Citation: Fisher, Mark; Zhang, Jingjing; Taylor, Nicolas; Howard, Mark; Berkowitz, Oliver; Debowski, Aleksandra; Behsaz, Bahar; Whelan, James; Pevzner, Pavel; Mylne, Joshua. "A family of small, cyclic peptides buried in preproalbumin since the Eocene epoch"  Plant Direct 2, e00042-e00042 (2018).
PubMed: 30417166

Assembly members:

Assembly members:
GLY-LEU-LEU-GLY-ILE-THR-ASP, polymer, 7 residues, 687.784 Da.

Natural source:

Natural source:   Common Name: Senecio pinnatifolius   Taxonomy ID: 904569   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Senecio pinnatifolius

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GLY-LEU-LEU-GLY-ILE-THR-ASP: GLLGITD

Data sets:
Data typeCount
13C chemical shifts14
1H chemical shifts45

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 7 residues - 687.784 Da.

1   GLYLEULEUGLYILETHRASP

Samples:

sample_1: PLP-4 1.0 ± 0.2 mg/mL

sample_conditions_1: ionic strength: 1.2 mM; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D ROESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSY 80sample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSY 20sample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4.2, CCPN - data analysis

TOPSPIN v3.5, Bruker Biospin - collection

YASARA v16.7.22, Elmar Krieger - refinement, structure calculation

NMR spectrometers:

  • Bruker AvanceIII 600 MHz