BMRB Entry 30313

Title:
SOLUTION NMR STRUCTURE OF SIGLEC-5 BINDING DOMAIN FROM STREPTOCOCCAL BETA PROTEIN
Deposition date:
2017-06-26
Original release date:
2018-06-21
Authors:
Eletsky, A.; Chen, C.; Fong, J.; Nizet, V.; Varki, A.; Prestegard, J.
Citation:

Citation: Eletsky, A.; Chen, C.; Fong, J.; Nizet, V.; Varki, A.; Prestegard, J.. "SOLUTION NMR STRUCTURE OF SIGLEC-5 BINDING DOMAIN FROM STREPTOCOCCAL BETA PROTEIN"  .

Assembly members:

Assembly members:
entity_1, polymer, 99 residues, 11513.662 Da.

Natural source:

Natural source:   Common Name: Streptococcus agalactiae   Taxonomy ID: 1311   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus agalactiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Data typeCount
13C chemical shifts436
15N chemical shifts123
1H chemical shifts722

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 99 residues - 11513.662 Da.

1   GLYVALGLULYSTHRALAGLYGLUTHRSER
2   ALATHRASPTHRGLYLYSARGGLULYSGLN
3   LEUGLNGLNTRPLYSASNASNLEULYSASN
4   ASPVALASPASNTHRILELEUSERHISGLU
5   GLNLYSASNGLUPHELYSTHRLYSILEASP
6   GLUTHRASNASPSERASPALALEULEUGLU
7   LEUGLUASNGLNPHEASNGLUTHRASNARG
8   LEULEUHISILELYSGLNHISGLUGLUVAL
9   GLULYSASPLYSLYSALALYSGLNGLNLYS
10   THRLEULYSGLNSERASPTHRLYSVAL

Samples:

sample_1: DSS 4 uM; IgA-Fc Receptor, [U-13C; U-15N], 0.45 mM; sodium azide 0.01%; sodium chloride 100 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 130 mM; pH: 6.5; pressure: 1 bar; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C CT-HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C CT-HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSY aliphaticsample_1isotropicsample_conditions_1
3D HCCH-COSY aromaticsample_1isotropicsample_conditions_1
3D HCCH-COSY aliphaticsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D (H)CA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CARA v1.9.1.7, Keller and Wuthrich - chemical shift assignment, peak picking

CNS, Brunger A. T. et.al. - refinement

CYANA v3.97, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRPipe v8.9, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ v4.2A, Varian - collection

NMR spectrometers:

  • Agilent DD2 600 MHz
  • Varian VNMRS 600 MHz
  • Agilent DD2 900 MHz
  • Agilent DD2 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks