BMRB Entry 30296

Title:
Solution NMR structure of the HMG domain of human FACT complex subunit SSRP1
Deposition date:
2017-05-21
Original release date:
2018-05-17
Authors:
Hu, Q.; Botuyan, M.; Mer, G.
Citation:

Citation: Hu, Q.; Botuyan, M.; Mer, G.. "Solution NMR structure of the HMG domain of human FACT complex subunit SSRP1"  .

Assembly members:

Assembly members:
entity_1, polymer, 69 residues, 8050.128 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTEV

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts269
15N chemical shifts68
1H chemical shifts453

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 69 residues - 8050.128 Da.

1   GLYHISMETSERALATYRMETLEUTRPLEU
2   ASNALASERARGGLULYSILELYSSERASP
3   HISPROGLYILESERILETHRASPLEUSER
4   LYSLYSALAGLYGLUILETRPLYSGLYMET
5   SERLYSGLULYSLYSGLUGLUTRPASPARG
6   LYSALAGLUASPALAARGARGASPTYRGLU
7   LYSALAMETLYSGLUTYRGLUGLYGLY

Samples:

sample_1: Human SSRP1 HMG Domain, [U-100% 13C; U-100% 15N], 0.6 mM; NaCl 50 mM; Sodium phosphate buffer 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.9; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D CCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - chemical shift assignment, data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

TOPSPIN, Bruker Biospin - collection

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMR spectrometers:

  • Bruker AvanceIII 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks