BMRB Entry 30229

Title:
Solution NMR Structure of Lasso Peptide Acinetodin
Deposition date:
2017-01-12
Original release date:
2017-02-02
Authors:
Bushin, L.; Metelev, M.; Severinov, K.; Seyedsayamdost, M.
Citation:

Citation: Metelev, M.; Arseniev, A.; Bushin, L.; Kuznedelov, K.; Artamonova, T.; Kondratenko, R.; Khodorkovskii, M.; Seyedsayamdost, M.; Severinov, K.. "Acinetodin and Klebsidin, RNA Polymerase Targeting Lasso Peptides Produced by Human Isolates of Acinetobacter gyllenbergii and Klebsiella pneumoniae."  ACS Chem. Biol. 12, 814-824 (2017).
PubMed: 28106375

Assembly members:

Assembly members:
Acinetodin, polymer, 18 residues, 1976.127 Da.

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 1217657   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Acinetobacter gyllenbergii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BW25113

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Acinetodin: GGKGPIFETWVTEGNYYG

Data sets:
Data typeCount
1H chemical shifts114

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 18 residues - 1976.127 Da.

1   GLYGLYLYSGLYPROILEPHEGLUTHRTRP
2   VALTHRGLUGLYASNTYRTYRGLY

Samples:

sample_1: Acinetodin, NA, 10 mg/mL; H2O 90%; D2O 10%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1anisotropicsample_conditions_1
2D 1H-1H TOCSYsample_1anisotropicsample_conditions_1
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1
2D 1H-13C HSQCsample_1anisotropicsample_conditions_1
2D 1H-13C HMBCsample_1anisotropicsample_conditions_1
1D 1Hsample_1anisotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

MestReNova, Mestrelab - data analysis

NMR spectrometers:

  • Bruker AvanceIII 800 MHz