BMRB Entry 30123

Title:
Connexin 32 G12R N-Terminal Mutant
Deposition date:
2016-06-21
Original release date:
2016-09-23
Authors:
Dowd, T.; Barigello, T.
Citation:

Citation: Batir, Y.; Bargiello, T.; Dowd, T.. "Structural studies of N-terminal mutants of Connexin 26 and Connexin 32 using (1)H NMR spectroscopy."  Arch. Biochem. Biophys. 608, 8-19 (2016).
PubMed: 27378082

Assembly members:

Assembly members:
entity_1, polymer, 23 residues, 2576.977 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XMNWTGLYTLLSRVNRHSTA IGR

Data sets:
Data typeCount
13C chemical shifts42
1H chemical shifts141

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 23 residues - 2576.977 Da.

1   ACEMETASNTRPTHRGLYLEUTYRTHRLEU
2   LEUSERARGVALASNARGHISSERTHRALA
3   ILEGLYARG

Samples:

sample_1: entity_1 1.0 ± 0.1 mM; KCl 100 ± 0.1 mM; TSP 100 ± 2 uM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

VNMR, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 600 MHz