BMRB Entry 30106

Title:
Phenol-soluble modulin Alpha 3
Deposition date:
2016-06-13
Original release date:
2016-08-26
Authors:
Towle, Kaitlyn; Lohans, Chris; Acedo, Jeella; van Belkum, Marco; Miskolzie, Mark; Vederas, John
Citation:

Citation: Towle, Kaitlyn; Lohans, Chris; Acedo, Jeella; van Belkum, Marco; Miskolzie, Mark; Vederas, John. "Solution Structures of Phenol-Soluble Modulins alpha 1, alpha 3, and beta 2, Virulence Factors from Staphylococcus aureus."  Biochemistry 55, 4798-4806 (2016).
PubMed: 27525453

Assembly members:

Assembly members:
Phenol-soluble modulin alpha 3 peptide, polymer, 22 residues, 2639.159 Da.

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: 273036   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Phenol-soluble modulin alpha 3 peptide: XEFVAKLFKFFKDLLGKFLG NN

Data sets:
Data typeCount
1H chemical shifts119

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 22 residues - 2639.159 Da.

1   FMEGLUPHEVALALALYSLEUPHELYSPHE
2   PHELYSASPLEULEUGLYLYSPHELEUGLY
3   ASNASN

Samples:

sample_1: PSMalpha3, none, 0.75 mM

sample_conditions_1: pH: 7; pressure: 753 mmHg; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

VNMRJ v4.2A, Varian - collection

NMR spectrometers:

  • Varian INOVA 700 MHz