BMRB Entry 28038

Title:
1H, 13C, 15N chemical shift assignments of FKBP12 protein from the pathogenic fungi Candida auris
Deposition date:
2019-11-05
Original release date:
2020-02-26
Authors:
Bashir, Qamar; LeMaster, David; Hernandez, Griselda
Citation:

Citation: Bashir, Qamar; LeMaster, David; Hernandez, Griselda. "1H, 13C, 15 N chemical shift assignments of the FKBP12 protein from the pathogenic fungi Candida auris and Candida glabrata"  Biomol. NMR Assignments 14, 105-109 (2020).
PubMed: 31950462

Assembly members:

Assembly members:
FKBP12, polymer, 111 residues, 11807.46 Da.

Natural source:

Natural source:   Common Name: budding yeasts   Taxonomy ID: 498019   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Candida auris

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11-a

Data sets:
Data typeCount
13C chemical shifts491
15N chemical shifts107
1H chemical shifts756

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FKBP121

Entities:

Entity 1, FKBP12 111 residues - 11807.46 Da.

1   METALAPROASNTHRTHRGLUVALGLUILE
2   ILESERGLUGLYASPGLYLYSVALPHEPRO
3   LYSVALGLYASPTHRVALTHRILEHISTYR
4   THRGLYTHRLEUGLUASNGLYLYSLYSPHE
5   ASPSERSERARGASPARGGLYLYSPROPHE
6   GLNCYSTHRILEGLYVALGLYHISVALILE
7   LYSGLYTRPASPILEGLYILEPROLYSLEU
8   SERVALGLYSERGLNALALYSLEUTHRILE
9   PROGLYHISGLUALATYRGLYSERARGGLY
10   PHEPROGLYLEUILEPROPROASPALATHR
11   LEUILEPHEASPVALGLULEULEUGLYVAL
12   ASN

Samples:

sample_1: FKBP12, [U-99% 15N], 1 mM; DTT 2 mM; TCEP 2 mM; sodium phosphate 25 mM

sample_2: FKBP12, [U-99% 13C; U-99% 15N], 1 mM; DTT 2 mM; TCEP 2 mM; sodium phosphate 25 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298.1 K

sample_conditions_2: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298.1 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_2
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_2
3D HNCOsample_2isotropicsample_conditions_2
3D HN(CA)COsample_2isotropicsample_conditions_2
3D HNCACBsample_2isotropicsample_conditions_2
3D CBCA(CO)NHsample_2isotropicsample_conditions_2
3D HCCH-TOCSYsample_2isotropicsample_conditions_2

Software:

Felix vND, Felix NMR - chemical shift assignment

TOPSPIN v3.2.7, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance III 600 MHz

Related Database Links:

NCBI 498019

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks