BMRB Entry 28035

Title:
Solid-state NMR assignments of the human H3 in the Widom '601' nucleosome core particle
Deposition date:
2019-10-31
Original release date:
2020-01-07
Authors:
Shi, Xiangyan; Prasanna, Chinmayi; Nordenskiold, Lars
Citation:

Citation: Shi, Xiangyan; Prasanna, Chinmayi; Pervushin, Konstantin; Nordenskiold, Lars. "Solid-state NMR 13C, 15N assignments of human histone H3 in the nucleosome core particle"  Biomol. NMR Assignments 14, 99-104 (2020).

Assembly members:

Assembly members:
human_histone_H3, polymer, 135 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3a

Data sets:
Data typeCount
13C chemical shifts387
15N chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human histone H31

Entities:

Entity 1, human histone H3 135 residues - Formula weight is not available

1   ALAARGTHRLYSGLNTHRALAARGLYSSER
2   THRGLYGLYLYSALAPROARGLYSGLNLEU
3   ALATHRLYSALAALAARGLYSSERALAPRO
4   ALATHRGLYGLYVALLYSLYSPROHISARG
5   TYRARGPROGLYTHRVALALALEUARGGLU
6   ILEARGARGTYRGLNLYSSERTHRGLULEU
7   LEUILEARGLYSLEUPROPHEGLNARGLEU
8   VALARGGLUILEALAGLNASPPHELYSTHR
9   ASPLEUARGPHEGLNSERSERALAVALMET
10   ALALEUGLNGLUALACYSGLUALATYRLEU
11   VALGLYLEUPHEGLUASPTHRASNLEUCYS
12   ALAILEHISALALYSARGVALTHRILEMET
13   PROLYSASPILEGLNLEUALAARGARGILE
14   ARGGLYGLUARGALA

Samples:

sample_1: human histone H3, [U-100% 13C; U-100% 15N], 3.1 mg; histone H4 2.2 mg; human histone H2A 2.7 mg; human histone H2B 2.7 mg; Widom 601 DNA 8.6 mg; H2O 35.9 mg

sample_conditions_1: ionic strength: 20 mM; pH: 7.5; pressure: 1 atm; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
2D CC DARR with 20 ms mixing timesample_1isotropicsample_conditions_1
2D CC DARR with 100 ms mixing timesample_1isotropicsample_conditions_1
2D NCAsample_1isotropicsample_conditions_1
2D NCOsample_1isotropicsample_conditions_1
3D NCACX with 75 ms CC DARRsample_1isotropicsample_conditions_1
3D NCOCX with 75 ms CC DARRsample_1isotropicsample_conditions_1
3D CANCOsample_1isotropicsample_conditions_1
2D CC DREAMsample_1isotropicsample_conditions_1
2D NcaCXsample_1isotropicsample_conditions_1
3D NCACX with 20 ms CC DARRsample_1isotropicsample_conditions_1
3D NCOCX with 20 ms CC DARRsample_1isotropicsample_conditions_1

Software:

SPARKY v3.116, Goddard - data analysis

TOPSPIN v3.5pl6, Bruker Biospin - collection

NMRPipe v3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz