BMRB Entry 28027

Name: human_CIRBP_68-137
Published: 2020-04-03

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28027

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

human_CIRBP_68-137

Deposition date:

2019-10-04

Original release date:

2020-04-03

Authors:

Bourgeois, Benjamin; Madl, Tobias

Citation:

Citation: Bourgeois, Benjamin; Hutten, Saskia; Gottschalk, Benjamin; Hofweber, Mario; Richter, Gesa; Sternat, Julia; Abou-Ajram, Claudia; Gobl, Christoph; Leitinger, Gerd; Graier, Wolfgang; Dormann, Dorothee; Madl, Tobias. "Nonclassical nuclear localization signals mediate nuclear import of CIRBP" Proc. Natl. Acad. Sci. U.S.A. 117, 8503-8514 (2020).
PubMed: 32234784

Assembly members:

Assembly members:
CIRBP, polymer, 98 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CIRBP: MKHHHHHHPMSDYDIPTTEN LYFQGAMGNGKSVDGRQIRV DQAGKSSDNRSRGYRGGSAG GRGFFRGGRGRGRGFSRGGG DRGYGGNRFESRSGGYGG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	81
1H chemical shifts	81

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CIRBP_68-137	1

Entities:

Entity 1, CIRBP_68-137 98 residues - Formula weight is not available

1

MET

LYS

HIS

PRO

MET

2

SER

ASP

TYR

ASP

ILE

PRO

THR

GLU

ASN

3

LEU

TYR

PHE

GLN

GLY

ALA

MET

GLY

ASN

GLY

4

LYS

SER

VAL

ASP

GLY

ARG

GLN

ILE

ARG

VAL

5

ASP

GLN

ALA

GLY

LYS

SER

ASP

ASN

ARG

6

SER

ARG

GLY

TYR

ARG

GLY

SER

ALA

GLY

7

GLY

ARG

GLY

PHE

ARG

GLY

ARG

GLY

8

ARG

GLY

ARG

GLY

PHE

SER

ARG

GLY

9

ASP

ARG

GLY

TYR

GLY

ASN

ARG

PHE

GLU

10

SER

ARG

SER

GLY

TYR

GLY

Samples:

sample_1: CIRBP, [U-99% 13C; U-99% 15N], 500 uM; D2O, [U-100% 2H], 10%; TRIS 50 mM; sodium chloride 150 mM; TCEP 2 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)NNH	sample_1	isotropic	sample_conditions_1

Software:

CCPN, Bruker Biospin, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - collection, peak picking, processing

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks