BMRB Entry 28026

Title:
TNPO1_328-381
Deposition date:
2019-10-04
Original release date:
2020-04-03
Authors:
bourgeois, benjamin; madl, tobias
Citation:

Citation: Bourgeois, Benjamin; Hutten, Saskia; Gottschalk, Benjamin; Hofweber, Mario; Richter, Gesa; Sternat, Julia; Abou-Ajram, Claudia; Gobl, Christoph; Leitinger, Gerd; Graier, Wolfgang; Dormann, Dorothee; Madl, Tobias. "Nonclassical nuclear localization signals mediate nuclear import of CIRBP"  Proc. Natl. Acad. Sci. U.S.A. 117, 8503-8514 (2020).
PubMed: 32234784

Assembly members:

Assembly members:
TNPO1, polymer, 57 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM-11

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts46
1H chemical shifts45

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TNPO1_328-3811

Entities:

Entity 1, TNPO1_328-381 57 residues - Formula weight is not available

1   GLYALAMETGLYASPVALGLUGLUASPGLU
2   THRILEPROASPSERGLUGLNASPILEARG
3   PROARGPHEHISARGSERARGTHRVALALA
4   GLNGLNHISASPGLUASPGLYILEGLUGLU
5   GLUASPASPASPASPASPGLUILEASPASP
6   ASPASPTHRILESERASPTRP

Samples:

sample_1: TNPO1, [U-99% 13C; U-99% 15N], 500 uM; D2O 10%; TRIS 50 mM; sodium chloride 150 mM; TCEP 2 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)NNHsample_1isotropicsample_conditions_1

Software:

CCPN, Bruker Biospin, CCPN, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - collection, peak picking, processing

NMR spectrometers:

  • GE Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks