BMRB Entry 28019

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for mouse Mdm2 (residues 282 to 432)
Published: 2019-10-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR28019

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for mouse Mdm2 (residues 282 to 432)

Deposition date:

2019-09-24

Original release date:

2019-10-15

Authors:

Theillet, Francois-Xavier

Citation:

Citation: Alik, Ania; Bouguechtouli, Chafiaa; Julien, Manon; Bermel, Wolfgang; Ghouil, Rania; Zinn-Justin, Sophie; Theillet, Francois-Xavier. "Sensitivity-Enhanced 13 C-NMR Spectroscopy for Monitoring Multisite Phosphorylation at Physiological Temperature and pH" Angew. Chem. Int. Ed. Engl. 59, 10411-10415 (2020).
PubMed: 32181947

Assembly members:

Assembly members:
Mdm2, polymer, 151 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Mdm2: GESDTDSFEGDPEISLADYW KCTSCNEMNPPLPSHCKRCW TLRENWLPDDKGKDKVEISE KAKLENSAQAEEGLDVPDGK KLTENDAKEPAAEEDSEEKA EQTPLSQESDDYSQPSTSSS IVYSSQESVKELKEETQDKD ESVESSFSLNA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	408
15N chemical shifts	129
1H chemical shifts	129

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Mdm2	1
2	Zn2+	2

Entities:

Entity 1, Mdm2 151 residues - Formula weight is not available

1

GLY

GLU

SER

ASP

THR

ASP

SER

PHE

GLU

GLY

2

ASP

PRO

GLU

ILE

SER

LEU

ALA

ASP

TYR

TRP

3

LYS

CYS

THR

SER

CYS

ASN

GLU

MET

ASN

PRO

4

PRO

LEU

PRO

SER

HIS

CYS

LYS

ARG

CYS

TRP

5

THR

LEU

ARG

GLU

ASN

TRP

LEU

PRO

ASP

6

LYS

GLY

LYS

ASP

LYS

VAL

GLU

ILE

SER

GLU

7

LYS

ALA

LYS

LEU

GLU

ASN

SER

ALA

GLN

ALA

8

GLU

GLY

LEU

ASP

VAL

PRO

ASP

GLY

LYS

9

LYS

LEU

THR

GLU

ASN

ASP

ALA

LYS

GLU

PRO

10

ALA

GLU

ASP

SER

GLU

LYS

ALA

11

GLU

GLN

THR

PRO

LEU

SER

GLN

GLU

SER

ASP

12

ASP

TYR

SER

GLN

PRO

SER

THR

SER

13

ILE

VAL

TYR

SER

GLN

GLU

SER

VAL

LYS

14

GLU

LEU

LYS

GLU

THR

GLN

ASP

LYS

ASP

15

GLU

SER

VAL

GLU

SER

PHE

SER

LEU

ASN

16

ALA

Entity 2, Zn2+ - 65.409 Da.

1

ZN

Samples:

sample_1: Mdm2, [U-99% 13C; U-99% 15N], 500 uM; ZN 1 mM

sample_conditions_1: ionic strength: 70 mM; pH: 6.85; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D (H)N(CA)NNH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.0.4, Bruker Biospin - collection, processing

ccpnmr_analysis, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks