BMRB Entry 28017

Title:
QBP1+TDP-43 POLYPEPTIDE
Deposition date:
2019-09-15
Original release date:
2019-10-03
Authors:
Laurents, Douglas; Mompean, Miguel
Citation:

Citation: Mompean, Miguel; Ramirez de Mingo, Daniel; Hervas, Ruben; Fernandez-Ramirez, Maria Del Carmen; Carrion-Vazquez, Mariano; Laurents, Douglas. "Molecular mechanism of the inhibition of TDP-43 amyloidogenesis by QBP1"  Arch. Biochem. Biophys. 675, 108113-108113 (2019).
PubMed: 31568752

Assembly members:

Assembly members:
QBP1+TDP-43, polymer, 28 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis   Vector: n.a.

Entity Sequences (FASTA):

Entity Sequences (FASTA):
QBP1+TDP-43: WKWWPGIFGGGASQQNQSGP SGNNQNQG

Data sets:
Data typeCount
13C chemical shifts64
15N chemical shifts37
1H chemical shifts176

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1QBP1+TDP-431

Entities:

Entity 1, QBP1+TDP-43 28 residues - Formula weight is not available

1   TRPLYSTRPTRPPROGLYILEPHEGLYGLY
2   GLYALASERGLNGLNASNGLNSERGLYPRO
3   SERGLYASNASNGLNASNGLNGLY

Samples:

sample_1: QBP1+TDP-43 0.94 ± 0.03 mM; potassium hydrogen phosphate 10 mM

sample_2: QBP1+TDP-43 0.94 ± 0.03 mM; potassium hydrogen phosphate 10 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 6.8; pressure: 1 atm; temperature: 278.2 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks