BMRB Entry 28001

Name: Structural basis for client recognition and activity of hsp40 chaperones
Published: 2019-11-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28001

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural basis for client recognition and activity of hsp40 chaperones

Deposition date:

2019-08-16

Original release date:

2019-11-07

Authors:

Jiang, Yajun; Rossi, Paolo; Kalodimos, Charalampos

Citation:

Citation: Jiang, Yajun; Rossi, Paolo; Kalodimos, Charalampos. "Structural basis for client recognition and activity of Hsp40 chaperones" Science 365, 1313-1319 (2019).
PubMed: 31604242

Assembly members:

Assembly members:
CbpA_SBD, polymer, 164 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CbpA_SBD: TRGHDIEIEVAVFLEETLTE HKRTISYNLPVYNAFGMIEQ EIPKTLNVKIPAGVGNGQRI RLKGQGTPGENGGPNGDLWL VIHIAPHPLFDIVGQDLEIV VPVSPWEAALGAKVTVPTLK ESILLTIPPGSQAGQRLRVK GKGLVSKKQTGDLYAVLKIV MPPK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	127
1H chemical shifts	127

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CbpA_SBD	1

Entities:

Entity 1, CbpA_SBD 164 residues - Formula weight is not available

1

THR

ARG

GLY

HIS

ASP

ILE

GLU

ILE

GLU

VAL

2

ALA

VAL

PHE

LEU

GLU

THR

LEU

THR

GLU

3

HIS

LYS

ARG

THR

ILE

SER

TYR

ASN

LEU

PRO

4

VAL

TYR

ASN

ALA

PHE

GLY

MET

ILE

GLU

GLN

5

GLU

ILE

PRO

LYS

THR

LEU

ASN

VAL

LYS

ILE

6

PRO

ALA

GLY

VAL

GLY

ASN

GLY

GLN

ARG

ILE

7

ARG

LEU

LYS

GLY

GLN

GLY

THR

PRO

GLY

GLU

8

ASN

GLY

PRO

ASN

GLY

ASP

LEU

TRP

LEU

9

VAL

ILE

HIS

ILE

ALA

PRO

HIS

PRO

LEU

PHE

10

ASP

ILE

VAL

GLY

GLN

ASP

LEU

GLU

ILE

VAL

11

VAL

PRO

VAL

SER

PRO

TRP

GLU

ALA

LEU

12

GLY

ALA

LYS

VAL

THR

VAL

PRO

THR

LEU

LYS

13

GLU

SER

ILE

LEU

THR

ILE

PRO

GLY

14

SER

GLN

ALA

GLY

GLN

ARG

LEU

ARG

VAL

LYS

15

GLY

LYS

GLY

LEU

VAL

SER

LYS

GLN

THR

16

GLY

ASP

LEU

TYR

ALA

VAL

LEU

LYS

ILE

VAL

17

MET

PRO

LYS

Samples:

sample_1: CbpA_SBD, [U-13C; U-15N; U-2H], 300 uM; potassium phosphate 20 mM; potassium chloride 75 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - data analysis

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks