BMRB Entry 27998

Name: Structural basis for client recognition and activity of Hsp40 chaperones
Published: 2019-11-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27998

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural basis for client recognition and activity of Hsp40 chaperones

Deposition date:

2019-08-16

Original release date:

2019-11-07

Authors:

Jiang, Yajun; Rossi, Paolo; Kalodimos, Charalampos

Citation:

Citation: Jiang, Yajun; Rossi, Paolo; Kalodimos, Charalampos. "Structural basis for client recognition and activity of Hsp40 chaperones" Science 365, 1313-1319 (2019).
PubMed: 31604242

Assembly members:

Assembly members:
DnaJB1_SBD, polymer, 185 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DnaJB1_SBD: ARKKQDPPVTHDLRVSLEEI YSGCTKKMKISHKRLNPDGK SIRNEDKILTIEVKKGWKEG TKITFPKEGDQTSNNIPADI VFVLKDKPHNIFKRDGSDVI YPARISLREALCGCTVNVPT LDGRTIPVVFKDVIRPGMRR KVPGEGLPLPKTPEKRGDLI IEFEVIFPERIPQTSRTVLE QVLPI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	148
1H chemical shifts	148

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DnaJB1_SBD	1

Entities:

Entity 1, DnaJB1_SBD 185 residues - Formula weight is not available

1

ALA

ARG

LYS

GLN

ASP

PRO

VAL

THR

2

HIS

ASP

LEU

ARG

VAL

SER

LEU

GLU

ILE

3

TYR

SER

GLY

CYS

THR

LYS

MET

LYS

ILE

4

SER

HIS

LYS

ARG

LEU

ASN

PRO

ASP

GLY

LYS

5

SER

ILE

ARG

ASN

GLU

ASP

LYS

ILE

LEU

THR

6

ILE

GLU

VAL

LYS

GLY

TRP

LYS

GLU

GLY

7

THR

LYS

ILE

THR

PHE

PRO

LYS

GLU

GLY

ASP

8

GLN

THR

SER

ASN

ILE

PRO

ALA

ASP

ILE

9

VAL

PHE

VAL

LEU

LYS

ASP

LYS

PRO

HIS

ASN

10

ILE

PHE

LYS

ARG

ASP

GLY

SER

ASP

VAL

ILE

11

TYR

PRO

ALA

ARG

ILE

SER

LEU

ARG

GLU

ALA

12

LEU

CYS

GLY

CYS

THR

VAL

ASN

VAL

PRO

THR

13

LEU

ASP

GLY

ARG

THR

ILE

PRO

VAL

PHE

14

LYS

ASP

VAL

ILE

ARG

PRO

GLY

MET

ARG

15

LYS

VAL

PRO

GLY

GLU

GLY

LEU

PRO

LEU

PRO

16

LYS

THR

PRO

GLU

LYS

ARG

GLY

ASP

LEU

ILE

17

ILE

GLU

PHE

GLU

VAL

ILE

PHE

PRO

GLU

ARG

18

ILE

PRO

GLN

THR

SER

ARG

THR

VAL

LEU

GLU

19

GLN

VAL

LEU

PRO

ILE

Samples:

sample_1: DnaJB1_SBD, [U-13C; U-15N; U-2H], 400 uM; potassium phosphate 20 mM; potassium chloride 75 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCOCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - data analysis

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks