BMRB Entry 27992

Title:
MYB28 116-197 chemical shifts
Deposition date:
2019-08-02
Original release date:
2019-10-29
Authors:
Millard, Peter; Bugge, Katrine; Kragelund, Birthe
Citation:

Citation: Millard, Peter; Bugge, Katrine; Marabini, Riccardo; Boomsma, Wouter; Burow, Meike; Kragelund, Birthe. "IDDomainSpotter: Compositional bias reveals domains in long disordered protein regions - insights from transcription factors"  Protein Sci. 29, 169-183 (2020).
PubMed: 31642121

Assembly members:

Assembly members:
MYB28_residues_116-197, polymer, 82 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T-1

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts190
15N chemical shifts63
1H chemical shifts61

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MYB28 residues 116-1971

Entities:

Entity 1, MYB28 residues 116-197 82 residues - Formula weight is not available

1   SERMETGLUGLNGLYILEASPPROVALTHR
2   HISLYSPROLEUALASERSERSERASNPRO
3   THRVALASPGLUASNLEUASNSERPROASN
4   ALASERSERSERASPLYSGLNTYRSERARG
5   SERSERSERMETPROPHELEUSERARGPRO
6   PROPROSERSERCYSASNMETVALSERLYS
7   VALSERGLULEUSERSERASNASPGLYTHR
8   PROILEGLNGLYSERSERLEUSERCYSLYS
9   LYSARG

Samples:

sample_1_MYB28_116-197: DSS 1 mM; TCEP 100 uM; sodium azide 0.03 % v/v; sodium phosphate 50 mM; MYB28 residues 116-197, [U-13C; U-15N], 350 uM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1_MYB28_116-197isotropicsample_conditions_1
3D HNCACBsample_1_MYB28_116-197isotropicsample_conditions_1
3D HNCOCACBsample_1_MYB28_116-197isotropicsample_conditions_1
3D HN(CO)CAsample_1_MYB28_116-197isotropicsample_conditions_1
3D HNCOsample_1_MYB28_116-197isotropicsample_conditions_1
HN(CA)NNHsample_1_MYB28_116-197isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - processing

CcpNmr, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance III 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks