BMRB Entry 27978

Name: Complete 1H, 13C, 15N resonance assignments of the Vpr binding region of hHR23A (residues 223-363)
Published: 2019-11-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27978

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Complete 1H, 13C, 15N resonance assignments of the Vpr binding region of hHR23A (residues 223-363)

Deposition date:

2019-07-17

Original release date:

2019-11-08

Authors:

Byeon, In-Ja; Jung, Jinwon; Byeon, Chang; Gronenborn, Angela

Citation:

Citation: Byeon, In-Ja; Jung, Jinwon; Byeon, Chang; DeLucia, Maria; Ahn, Jinwoo; Gronenborn, Angela. "Complete 1H, 13C, 15N resonance assignments and secondary structure of the Vpr binding region of hHR23A (residues 223-363)" Biomol. NMR Assign. 14, 13-17 (2020).
PubMed: 31463759

Assembly members:

Assembly members:
hHR23A, polymer, 149 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21 vector

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hHR23A: ATEAAGENPLEFLRDQPQFQ NMRQVIQQNPALLPALLQQL GQENPQLLQQISRHQEQFIQ MLNEPPGELADISDVEGEVG AIGEEAPQMNYIQVTPQEKE AIERLKALGFPESLVIQAYF ACEKNENLAANFLLSQNFDD ELEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	628
15N chemical shifts	165
1H chemical shifts	1017

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hHR23A(223-363) monomoer	1

Entities:

Entity 1, hHR23A(223-363) monomoer 149 residues - Formula weight is not available

1

ALA

THR

GLU

ALA

GLY

GLU

ASN

PRO

LEU

2

GLU

PHE

LEU

ARG

ASP

GLN

PRO

GLN

PHE

GLN

3

ASN

MET

ARG

GLN

VAL

ILE

GLN

ASN

PRO

4

ALA

LEU

PRO

ALA

LEU

GLN

LEU

5

GLY

GLN

GLU

ASN

PRO

GLN

LEU

GLN

6

ILE

SER

ARG

HIS

GLN

GLU

GLN

PHE

ILE

GLN

7

MET

LEU

ASN

GLU

PRO

GLY

GLU

LEU

ALA

8

ASP

ILE

SER

ASP

VAL

GLU

GLY

GLU

VAL

GLY

9

ALA

ILE

GLY

GLU

ALA

PRO

GLN

MET

ASN

10

TYR

ILE

GLN

VAL

THR

PRO

GLN

GLU

LYS

GLU

11

ALA

ILE

GLU

ARG

LEU

LYS

ALA

LEU

GLY

PHE

12

PRO

GLU

SER

LEU

VAL

ILE

GLN

ALA

TYR

PHE

13

ALA

CYS

GLU

LYS

ASN

GLU

ASN

LEU

ALA

14

ASN

PHE

LEU

SER

GLN

ASN

PHE

ASP

15

GLU

LEU

GLU

HIS

Samples:

sample_1: hHR23A(223-363), [U-99% 13C; U-99% 15N], 0.6 ± 0.03 mM; DSS, [U-100% 13C], 0.1 ± 0.01 mM; sodium phosphate 25 ± 1 mM; sodium chloride 50 ± 1 mM; sodium azide 0.02 ± 0.001 %; zinc sulphate 1 ± 0.05 mM; EDTA 1 ± 0.05 mM; TCEP 1 ± 0.05 mM

sample_2: hHR23A(223-363), [U-99% 13C; U-99% 15N], 1.2 ± 0.06 mM; DSS, [U-100% 13C], 0.1 ± 0.01 mM; sodium phosphate 25 ± 1 mM; sodium chloride 50 ± 1 mM; sodium azide 0.02 ± 0.001 %; zinc sulphate 1 ± 0.05 mM; EDTA 1 ± 0.05 mM; TCEP 1 ± 0.05 mM

sample_3: hHR23A(223-363), [U-99% 13C; U-99% 15N], 2.7 ± 0.1 mM; DSS, [U-100% 13C], 0.1 ± 0.01 mM; HEPES 10 ± 0.5 mM; sodium chloride 50 ± 1 mM; sodium azide 0.02 ± 0.001 %; zinc sulphate 1 ± 0.05 mM; EDTA 1 ± 0.05 mM; TCEP 1 ± 0.05 mM

sample_4: hHR23A(223-363), [U-99% 13C; U-99% 15N], 0.084 ± 0.05 mM; MES 10 ± 0.5 mM; sodium chloride 50 ± 1 mM; sodium azide 0.02 ± 0.001 %; DTT 2 ± 0.1 mM

sample_conditions_1: ionic strength: 0.050 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0.050 M; pH: 6.5; pressure: 1 atm; temperature: 291 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_3	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D C(CO)NH	sample_3	isotropic	sample_conditions_1
3D H(CCO)NH	sample_3	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_3	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_3	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_3	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_3	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_4	isotropic	sample_conditions_2
3D HNCACB	sample_4	isotropic	sample_conditions_2
3D HNCO	sample_4	isotropic	sample_conditions_2
3D HN(CA)CO	sample_4	isotropic	sample_conditions_2
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 900 MHz
Bruker Avance 800 MHz
Bruker Avance 700 MHz
Bruker Avance 600 MHz

UNP	P54725
AlphaFold	Q5M7Z1