BMRB Entry 27962

Title:
1H, 15N, and 13C chemical shifts of red abalone FITZAP-8D
Deposition date:
2019-06-28
Original release date:
2020-02-26
Authors:
Wilburn, Damien; Tuttle, Lisa; Klevit, Rachel; Swanson, Willie
Citation:

Citation: Wilburn, Damien; Tuttle, Lisa; Klevit, Rachel; Swanson, Willie. "Indirect sexual selection drives rapid sperm protein evolution in abalone"  Elife 8, e52628-e52628 (2019).
PubMed: 31868593

Assembly members:

Assembly members:
FITZAP-8D, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Red abalone   Taxonomy ID: 6454   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Haliotis rufescens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11d

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts77
15N chemical shifts26
1H chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FITZAP-8D1

Entities:

Entity 1, FITZAP-8D 64 residues - Formula weight is not available

1   GLYSERHISGLYARGTHRSERSERTYRASP
2   ASPASPASPASPASPASPASPGLUPROPRO
3   LYSASPLEUILETHRLYSASPVALILEGLU
4   ALAVALGLYGLYASNILELYSMETPHEGLN
5   ARGARGLYSARGSERALAASPLEUASPTYR
6   GLYGLYARGTYRGLYASNASPASPPROASP
7   SERTYRLEUGLU

Samples:

rFITZAP-8D: FITZAP-8D+ATCUN, [U-99% 13C; U-99% 15N], 1 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCrFITZAP-8Disotropicsample_conditions_1
2D 1H-13C HSQCrFITZAP-8Disotropicsample_conditions_1
3D CBCA(CO)NHrFITZAP-8Disotropicsample_conditions_1
3D HNCOrFITZAP-8Disotropicsample_conditions_1
3D HNCACBrFITZAP-8Disotropicsample_conditions_1
3D 1H-15N NOESYrFITZAP-8Disotropicsample_conditions_1
3D 1H-15N TOCSYrFITZAP-8Disotropicsample_conditions_1
3D HNHArFITZAP-8Disotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - chemical shift calculation

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks