BMRB Entry 27956

Title:
ILVM methyl chemical shifts for Pyrococcus furiosus Rad50 NBD (L828F) with unlabeled Mre11
Deposition date:
2019-06-24
Original release date:
2019-07-23
Authors:
Boswell, Zachary; Canny, Marella; Sang, Julie; Latham, Michael
Citation:

Citation: Boswell, Zachary; Canny, Marella; Buschmann, Tanner; Sang, Julie; Latham, Michael. "Adjacent mutations in the archaeal Rad50 ABC ATPase D-loop disrupt allosteric regulation of ATP hydrolysis through different mechanisms"  Nucleic Acids Res. 48, 2457-2472 (2020).
PubMed: 31889185

Assembly members:

Assembly members:
Pf_Rad50_NBD_(L828F_mutant)_A, polymer, 199 residues, Formula weight is not available
Pf_Rad50_NBD_(L828F_mutant)_B, polymer, 174 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 2261   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSET

Data sets:
Data typeCount
13C chemical shifts138
1H chemical shifts411

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rad50NBD (L828F), subunit A1
2Rad50NBD (L828F), subunit B2

Entities:

Entity 1, Rad50NBD (L828F), subunit A 199 residues - Formula weight is not available

1   ALAMETGLYSERMETLYSLEUGLUARGVAL
2   THRVALLYSASNPHEARGSERHISSERASP
3   THRVALVALGLUPHELYSGLUGLYILEASN
4   LEUILEILEGLYGLNASNGLYSERGLYLYS
5   SERSERLEULEUASPALAILELEUVALGLY
6   LEUTYRTRPPROLEUARGILELYSASPILE
7   LYSLYSASPGLUPHETHRLYSVALGLYALA
8   ARGASPTHRTYRILEASPLEUILEPHEGLU
9   LYSASPGLYTHRLYSTYRARGILETHRARG
10   ARGPHELEULYSGLYTYRSERSERGLYGLU
11   ILEHISALAMETLYSARGLEUVALGLYASN
12   GLUTRPLYSHISVALTHRGLUPROSERSER
13   LYSALAILESERALAPHEMETGLULYSLEU
14   ILEPROTYRASNILEPHELEUASNALAILE
15   TYRILEARGGLNGLYGLNILEASPALAILE
16   LEUGLUSERASPGLUALAARGGLULYSVAL
17   VALARGGLUVALLEUASNLEUASPLYSPHE
18   GLUTHRALATYRLYSLYSLEUSERGLULEU
19   LYSLYSTHRILEASNASNARGILELYSGLU
20   TYRARGASPILELEUALAARGTHRGLU

Entity 2, Rad50NBD (L828F), subunit B 174 residues - Formula weight is not available

1   ARGGLUARGVALLYSLYSGLUILELYSASP
2   LEUGLULYSALALYSASPPHETHRGLUGLU
3   LEUILEGLULYSVALLYSLYSTYRLYSALA
4   LEUALAARGGLUALAALALEUSERLYSILE
5   GLYGLULEUALASERGLUILEPHEALAGLU
6   PHETHRGLUGLYLYSTYRSERGLUVALVAL
7   VALARGALAGLUGLUASNLYSVALARGLEU
8   PHEVALVALTRPGLUGLYLYSGLUARGPRO
9   LEUTHRPHELEUSERGLYGLYGLUARGILE
10   ALALEUGLYLEUALAPHEARGLEUALAMET
11   SERLEUTYRLEUALAGLYGLUILESERLEU
12   LEUILELEUASPGLUPROTHRPROTYRPHE
13   ASPGLUGLUARGARGARGLYSLEUILETHR
14   ILEMETGLUARGTYRLEULYSLYSILEPRO
15   GLNVALILELEUVALSERHISASPGLUGLU
16   LEULYSASPALAALAASPHISVALILEARG
17   ILESERLEUGLUASNGLYSERSERLYSVAL
18   GLUVALVALSER

Samples:

sample_1: Pf Rad50 NBD (L828F mutant), U-2H, Iled1-[13CH3], Leud/Valg-[13CH3,12CD3], Mete-[13CH3] (ILVM), 0.5 mM; Pf Rad50 NBD (L828F mutant), U-2H, Iled1-[13CH3], Leud/Valg-[13CH3,12CD3], Mete-[13CH3] (ILVM), 0.5 mM; Pf Mre11 0.5 mM

sample_conditions_1: ionic strength: 250 mM; pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 13C,1H methyl-TROSY HMQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Agilent DD2 600 MHz