BMRB Entry 27953

Name: Sequence-specific assignments of Plasmodium falciparum PFE0055c J-domain
Published: 2019-09-23

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27953

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequence-specific assignments of Plasmodium falciparum PFE0055c J-domain

Deposition date:

2019-06-21

Original release date:

2019-09-23

Authors:

Vakonakis, Ioannis; Day, Jemma

Citation:

Citation: Day, Jemma; Passecker, Armin; Beck, Hans-Peter; Vakonakis, Ioannis. "The Plasmodium falciparum Hsp70-x chaperone assists the heat stress response of the malaria parasite" FASEB J. 33, 14611-14624 (2019).
PubMed: 31690116

Assembly members:

Assembly members:
PFE0055c-J, polymer, 74 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Plasmodium falciparum Taxonomy ID: 5833 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium falciparum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pFLOAT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PFE0055c-J: GPDETDYYAVLGLTKDCTQD DIKKAYRKLAMKWHPDKHLN DEDKVEAERKFKLIGEAYEV LSDEEKRKNYDLFG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	132
15N chemical shifts	65
1H chemical shifts	65

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	J-domain	1

Entities:

Entity 1, J-domain 74 residues - Formula weight is not available

Two residues (GP) from cloning artefact. Remaining corresponds to UniProt Q8I489 amino acids 77-148.

1

GLY

PRO

ASP

GLU

THR

ASP

TYR

ALA

VAL

2

LEU

GLY

LEU

THR

LYS

ASP

CYS

THR

GLN

ASP

3

ASP

ILE

LYS

ALA

TYR

ARG

LYS

LEU

ALA

4

MET

LYS

TRP

HIS

PRO

ASP

LYS

HIS

LEU

ASN

5

ASP

GLU

ASP

LYS

VAL

GLU

ALA

GLU

ARG

LYS

6

PHE

LYS

LEU

ILE

GLY

GLU

ALA

TYR

GLU

VAL

7

LEU

SER

ASP

GLU

LYS

ARG

LYS

ASN

TYR

8

ASP

LEU

PHE

GLY

Samples:

sample_1: PFE0055c-J, [U-99% 13C; U-99% 15N], 0.5 mM; D2O 5%; DSS 0.05 mM; DTT 2 mM; sodium chloride 150 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 0.38 M; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

NMR spectrometers:

Bruker Avance 750 MHz