BMRB Entry 27943

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments of reduced human DJ-1
Deposition date:
2019-06-14
Original release date:
2019-08-26
Authors:
Luchinat, Enrico; Barbieri, Letizia
Citation:

Citation: Barbieri, Letizia; Luchinat, Enrico. "Backbone resonance assignment of human DJ-1 in the reduced state and in the cysteine sulfinic acid state"  Biomol. NMR Assignments 13, 371-376 (2019).
PubMed: 31377986

Assembly members:

Assembly members:
DJ-1, polymer, 189 residues, 19846.94 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
13C chemical shifts540
15N chemical shifts175
1H chemical shifts175

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DJ-1 monomer reduced, 11
2DJ-1 monomer reduced, 21

Entities:

Entity 1, DJ-1 monomer reduced, 1 189 residues - 19846.94 Da.

Met1 is replaced by Ser1 as a leftover from the purification tag cleavage.

1   SERALASERLYSARGALALEUVALILELEU
2   ALALYSGLYALAGLUGLUMETGLUTHRVAL
3   ILEPROVALASPVALMETARGARGALAGLY
4   ILELYSVALTHRVALALAGLYLEUALAGLY
5   LYSASPPROVALGLNCYSSERARGASPVAL
6   VALILECYSPROASPALASERLEUGLUASP
7   ALALYSLYSGLUGLYPROTYRASPVALVAL
8   VALLEUPROGLYGLYASNLEUGLYALAGLN
9   ASNLEUSERGLUSERALAALAVALLYSGLU
10   ILELEULYSGLUGLNGLUASNARGLYSGLY
11   LEUILEALAALAILECYSALAGLYPROTHR
12   ALALEULEUALAHISGLUILEGLYPHEGLY
13   SERLYSVALTHRTHRHISPROLEUALALYS
14   ASPLYSMETMETASNGLYGLYHISTYRTHR
15   TYRSERGLUASNARGVALGLULYSASPGLY
16   LEUILELEUTHRSERARGGLYPROGLYTHR
17   SERPHEGLUPHEALALEUALAILEVALGLU
18   ALALEUASNGLYLYSGLUVALALAALAGLN
19   VALLYSALAPROLEUVALLEULYSASP

Samples:

sample_1: DJ-1, [U-13C; U-15N], 360 uM; DTT 5 mM; potassium phosphate 20 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

CARA v1.9.1.7, Fred Damberger, Rochus Keller - chemical shift assignment

NMR spectrometers:

  • Bruker Avance III 950 MHz
  • Bruker Avance NEO 500 MHz

Related Database Links:

GB D61380

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks