BMRB Entry 27922

Title:
scAtg3(del 1-18, 86-159, 248-278)
Deposition date:
2019-05-23
Original release date:
2019-08-06
Authors:
Royappa, Grace; Zheng, Yumei; Yu, Qiu; Schulman, Brenda
Citation:

Citation: Zheng, Yumei; Qiu, Yu; Grace, Christy; Liu, Xu; Klionsky, Daniel; Schulman, Brenda. "A switch element in the autophagy E2 Atg3 mediates allosteric regulation across the lipidation cascade"  Nat. Commun. 10, 3600-3600 (2019).
PubMed: 31399562

Assembly members:

Assembly members:
Atg3, polymer, 189 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Saccharomyces cerevisiae   Vector: pGEX-4T-1

Data sets:
Data typeCount
13C chemical shifts233
15N chemical shifts143
1H chemical shifts143

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Atg31

Entities:

Entity 1, Atg3 189 residues - Formula weight is not available

1   GLYSERLYSSERTHRPHELEUTHRTHRGLY
2   GLNILETHRPROGLUGLUPHEVALGLNALA
3   GLYASPTYRLEUALAHISMETPHEPROTHR
4   TRPLYSTRPASNGLUGLUSERSERASPILE
5   SERTYRARGASPPHELEUPROLYSASNLYS
6   GLNPHELEUILEILEARGLYSVALPROALA
7   ASPLYSARGALAGLUGLNALAVALGLUALA
8   LYSASPMETALAGLNGLUARGTYRTYRASP
9   LEUTYRILEALATYRSERTHRSERTYRARG
10   VALPROLYSMETTYRILEVALGLYPHEASN
11   SERASNGLYSERPROLEUSERPROGLUGLN
12   METPHEGLUASPILESERALAASPTYRARG
13   THRLYSTHRALATHRILEGLULYSLEUPRO
14   PHETYRLYSASNSERVALLEUSERVALSER
15   ILEHISPROCYSLYSHISALAASNVALMET
16   LYSILELEULEUASPLYSVALASPSERLEU
17   ARGVALASPGLNTYRLEUILEVALPHELEU
18   LYSPHEILETHRSERVALTHRPROSERILE
19   GLNHISASPTYRTHRMETGLUGLYTRP

Samples:

sample_1: Atg3, [U-100% 13C; U-100% 15N], 0.75 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - data analysis

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks