BMRB Entry 27904

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for human Fis1 residues 1-125
Deposition date:
2019-05-13
Original release date:
2019-06-13
Authors:
Egner, John; Peterson, Francis
Citation:

Citation: Egner, John; Jensen, Davin; Olp, Michael; Kennedy, Nolan; Volkman, Brian; Peterson, Francis; Smith, Brian; Hill, R. "Development and Validation of 2D Difference Intensity Analysis for Chemical Library Screening by Protein- Detected NMR Spectroscopy"  ChemBioChem 19, 448-458 (2018).
PubMed: 29239081

Assembly members:

Assembly members:
hFis1, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30

Data sets:
Data typeCount
13C chemical shifts465
15N chemical shifts118
1H chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hFis11

Entities:

Entity 1, hFis1 125 residues - Formula weight is not available

1   METGLUALAVALLEUASNGLULEUVALSER
2   VALGLUASPLEULEULYSPHEGLULYSLYS
3   PHEGLNSERGLULYSALAALAGLYSERVAL
4   SERLYSSERTHRGLNPHEGLUTYRALATRP
5   CYSLEUVALARGSERLYSTYRASNASPASP
6   ILEARGLYSGLYILEVALLEULEUGLUGLU
7   LEULEUPROLYSGLYSERLYSGLUGLUGLN
8   ARGASPTYRVALPHETYRLEUALAVALGLY
9   ASNTYRARGLEULYSGLUTYRGLULYSALA
10   LEULYSTYRVALARGGLYLEULEUGLNTHR
11   GLUPROGLNASNASNGLNALALYSGLULEU
12   GLUARGLEUILEASPLYSALAMETLYSLYS
13   ASPGLYLEUVALGLY

Samples:

sample_1: HEPES 20 mM; sodium chloride 175 mM; DTT 2 mM; sodium azide 0.02%; hFis1, [U-98% 13C; U-98% 15N], 0.96 mM

sample_conditions_1: ionic strength: 0.175 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

GARANT, Bartels, Guntert, Billeter and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance III 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks