BMRB Entry 27900

Title:
Identification of a short peptide motif in the N-terminal region of alpha-synuclein that plays a critical role in aggregation and function
Deposition date:
2019-05-13
Original release date:
2020-04-03
Authors:
Maya-Martinez, Roberto; Doherty, Ciaran; Ulamec, Sabine; Brockwell, David; Radford, Sheena
Citation:

Citation: Doherty, Ciaran; Ulamec, Sabine; Maya Martinez, Roberto Carlos; Good, Sarah; Makepeace, Jemma; Khan, Nasir; van Oosten-Hawle, Patricija; Radford, Sheena; Brockwell, David. "A short motif in the N-terminal region of alpha-synuclein is critical for both aggregation and function"  Nat. Struct. Mol. Biol. 27, 249-259 (2020).
PubMed: 32157247

Assembly members:

Assembly members:
Human_alpha-Synuclein, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET23a

Data sets:
Data typeCount
13C chemical shifts404
15N chemical shifts134
1H chemical shifts776

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human alpha-Synuclein1

Entities:

Entity 1, Human alpha-Synuclein 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLEUSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

h-a-Synuclein: Human alpha-Synuclein, [U-13C; U-15N], 500 uM; sodium chloride 20 mM; sodium acetate 20 mM

Low_salt_at_pH_4.5: pH: 4.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCh-a-SynucleinisotropicLow_salt_at_pH_4.5
2D 1H-13C HSQCh-a-SynucleinisotropicLow_salt_at_pH_4.5
3D HNCOh-a-SynucleinisotropicLow_salt_at_pH_4.5
3D HN(CA)COh-a-SynucleinisotropicLow_salt_at_pH_4.5
3D HNCACBh-a-SynucleinisotropicLow_salt_at_pH_4.5
3D HN(CO)CACBh-a-SynucleinisotropicLow_salt_at_pH_4.5
3D HN(CA)NHh-a-SynucleinisotropicLow_salt_at_pH_4.5
3D HN(CO)CACBh-a-SynucleinisotropicLow_salt_at_pH_4.5
3D HNNHh-a-SynucleinisotropicLow_salt_at_pH_4.5
3D 1H-15N TOCSYh-a-SynucleinisotropicLow_salt_at_pH_4.5
3D hCCH-TOCSYh-a-SynucleinisotropicLow_salt_at_pH_4.5
3D HcCH-TOCSYh-a-SynucleinisotropicLow_salt_at_pH_4.5

Software:

NMRPipe v2018.275.17.17, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN v3.5, Bruker Biospin - collection

CCPN vv.2.4.2, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

  • Oxford Oxford 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks