BMRB Entry 27878

Title:
K1-K9 fragments of beta2-microglobulin
Deposition date:
2019-04-16
Original release date:
2019-11-22
Authors:
Muta, Hiroya; So, Masatomo; Sakurai, Kazumasa; Goto, Yuji
Citation:

Citation: Muta, Hiroya; So, Masatomo; Sakurai, Kazumasa; Kardos, Jozsef; Naiki, Hironobu; Goto, Yuji. "Amyloid formation under complicated conditions in which beta2-microglobulin coexists with its proteolytic fragments"  Biochemistry 58, 4925-4934 (2019).
PubMed: 31724398

Assembly members:

Assembly members:
K1, polymer, 7 residues, Formula weight is not available
K2, polymer, 13 residues, Formula weight is not available
K4, polymer, 7 residues, Formula weight is not available
K5, polymer, 10 residues, Formula weight is not available
K7, polymer, 16 residues, Formula weight is not available
K8, polymer, 3 residues, Formula weight is not available
K9, polymer, 5 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pColdB2M

Entity Sequences (FASTA):

Entity Sequences (FASTA):
K1: MIQRTPK
K2: IQVYSRHPAENGK
K4: NGERIEK
K5: VEHSDLSFSK
K7: DEYACRVNHVTLSQPK
K8: IVK
K9: WDRDM

Data sets:
Data typeCount
13C chemical shifts60
15N chemical shifts50
1H chemical shifts50

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1K11
2K22
3K43
4K54
5K75
6K86
7K97

Entities:

Entity 1, K1 7 residues - Formula weight is not available

1   METILEGLNARGTHRPROLYS

Entity 2, K2 13 residues - Formula weight is not available

1   ILEGLNVALTYRSERARGHISPROALAGLU
2   ASNGLYLYS

Entity 3, K4 7 residues - Formula weight is not available

1   ASNGLYGLUARGILEGLULYS

Entity 4, K5 10 residues - Formula weight is not available

1   VALGLUHISSERASPLEUSERPHESERLYS

Entity 5, K7 16 residues - Formula weight is not available

1   ASPGLUTYRALACYSARGVALASNHISVAL
2   THRLEUSERGLNPROLYS

Entity 6, K8 3 residues - Formula weight is not available

1   ILEVALLYS

Entity 7, K9 5 residues - Formula weight is not available

1   TRPASPARGASPMET

Samples:

sample_1: K1 peptides, [U-99% 13C; U-99% 15N], 50 uM; K2 peptides, [U-99% 13C; U-99% 15N], 50 uM; K4 peptides, [U-99% 13C; U-99% 15N], 50 uM; K5 peptides, [U-99% 13C; U-99% 15N], 50 uM; K7 peptides, [U-99% 13C; U-99% 15N], 50 uM; K8 peptides, [U-99% 13C; U-99% 15N], 50 uM; K9 peptides, [U-99% 13C; U-99% 15N], 50 uM; HCl 10 mM; sodium chloride 100 mM; D2O, [U-99% 2H], 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 1.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks