BMRB Entry 27835

Name: HRdup moPrP 118-231
Published: 2019-05-22

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27835

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

HRdup moPrP 118-231

Deposition date:

2019-03-12

Original release date:

2019-05-22

Authors:

Fu, Ze-Lin; Holmes, Peter; Westaway, David; Sykes, Brian

Citation:

Citation: Fu, Ze-Lin; Holmes, Peter; Westaway, David; Sykes, Brian. "Nascent beta structure in the Elongated Hydrophobic Region of a Gerstmann-Straussler-Scheinker PrP Allele" J. Mol. Biol. 431, 2599-2611 (2019).
PubMed: 31034890

Assembly members:

Assembly members:
HRdup_mPrP, polymer, 143 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pD444

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HRdup_mPrP: HHHHHHGASTGGQQGENLYF QGAVVGGLGGYVLGGLGGYV LGSAMSRPMIHFGNDWEDRY YRENMYRYPNQVYYRPVDQY SNQNNFVHDCVNITIKQHTV TTTTKGENFTETDVKMMERV VEQMCVTQYQKESQAYYDGR RSS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	169
15N chemical shifts	106
1H chemical shifts	358

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HRduo mPrP	1

Entities:

Entity 1, HRduo mPrP 143 residues - Formula weight is not available

1

HIS

GLY

ALA

SER

THR

2

GLY

GLN

GLY

GLU

ASN

LEU

TYR

PHE

3

GLN

GLY

ALA

VAL

GLY

LEU

GLY

4

TYR

VAL

LEU

GLY

LEU

GLY

TYR

VAL

5

LEU

GLY

SER

ALA

MET

SER

ARG

PRO

MET

ILE

6

HIS

PHE

GLY

ASN

ASP

TRP

GLU

ASP

ARG

TYR

7

TYR

ARG

GLU

ASN

MET

TYR

ARG

TYR

PRO

ASN

8

GLN

VAL

TYR

ARG

PRO

VAL

ASP

GLN

TYR

9

SER

ASN

GLN

ASN

PHE

VAL

HIS

ASP

CYS

10

VAL

ASN

ILE

THR

ILE

LYS

GLN

HIS

THR

VAL

11

THR

LYS

GLY

GLU

ASN

PHE

THR

12

GLU

THR

ASP

VAL

LYS

MET

GLU

ARG

VAL

13

VAL

GLU

GLN

MET

CYS

VAL

THR

GLN

TYR

GLN

14

LYS

GLU

SER

GLN

ALA

TYR

ASP

GLY

ARG

15

ARG

SER

Samples:

sample_1: HRdup mPrP, [U-99% 13C; U-99% 15N], 0.1 mM; sodium acetate 10 mM; DSS 0.5 mM

sample_conditions_1: ionic strength: 0.01 M; pH: 5.0; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ v3.2, Varian - collection

NMR spectrometers:

Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks