BMRB Entry 27834

Title:
Sequence Specific Assignment of OSR1 C-terminal Domain.
Deposition date:
2019-03-12
Original release date:
2019-05-22
Authors:
AlAmri, Mubarak; Jeeves, Mark; Mehellou, Youcef
Citation:

Citation: AlAmri, Mubarak; Jeeves, Mark; Mehellou, Youcef. "Sequence Specific Assignment and Determination of OSR1 C-terminal Domain Structure by NMR"  Biochem. Biophys. Res. Commun. 512, 338-343 (2019).
PubMed: 30894278

Assembly members:

Assembly members:
OSR1, polymer, 95 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-1

Data sets:
Data typeCount
13C chemical shifts247
15N chemical shifts80
1H chemical shifts80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal Domain1

Entities:

Entity 1, C-terminal Domain 95 residues - Formula weight is not available

1   PROILESERLEUVALLEUARGLEUARGASN
2   SERLYSLYSGLULEUASNASPILEARGPHE
3   GLUPHETHRPROGLYARGASPTHRALAGLU
4   GLYVALSERGLNGLULEUILESERALAGLY
5   LEUVALASPGLYARGASPLEUVALILEVAL
6   ALAALAASNLEUGLNLYSILEVALGLUGLU
7   PROGLNSERASNARGSERVALTHRPHELYS
8   LEUALASERGLYVALGLUGLYSERASPILE
9   PROASPASPGLYLYSLEUILEGLYPHEALA
10   GLNLEUSERILESER

Samples:

sample_1: OSR1, [U-100% 13C; U-100% 15N], 0.2 mM; TRIS, [U-2H], 50 mM; sodium chloride 150 mM; DTT 1 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

CcpNmr_Analysis, ranken, Boucher, Stevens, Fogh, Pajon, Llinas, Ulrich, Markley, Ionides, Laue - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks