BMRB Entry 27724

Title:
NMR Assignments of the apo-form of Aedes aegypti odorant binding protein 22
Deposition date:
2018-12-11
Original release date:
2018-12-19
Authors:
Jones, David; Wang, Jing
Citation:

Citation: Wang, Jing; Murphy, Emma; Nix, Jay; Jones, David. "Aedes aegypti Odorant Binding Protein 22 selectively binds fatty acids through a conformational change in its C-terminal tail"  Sci. Rep. 10, 3300-3300 (2020).
PubMed: 32094450

Assembly members:

Assembly members:
OBP22, polymer, 123 residues, 14379.2591 Da.

Natural source:

Natural source:   Common Name: Yellow Fever Mosquito   Taxonomy ID: not available   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Aedes aegypti

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Aedes aegypti   Vector: pET13a

Data sets:
Data typeCount
13C chemical shifts241
15N chemical shifts128
1H chemical shifts138

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Aedes aegypti OBP221

Entities:

Entity 1, Aedes aegypti OBP22 123 residues - 14379.2591 Da.

Residue 1 is initiator methionine. This fragment represent the mature processed protein without the signal peptide.

1   METGLUPHETHRVALSERTHRTHRGLUASP
2   LEUGLNARGTYRARGTHRGLUCYSVALSER
3   SERLEUASNILEPROALAASPTYRVALGLU
4   LYSPHELYSLYSTRPGLUPHEPROGLUASP
5   ASPTHRTHRMETCYSTYRILELYSCYSVAL
6   PHEASNLYSMETGLNLEUPHEASPASPTHR
7   GLUGLYPROLEUVALASPASNLEUVALHIS
8   GLNLEUALAHISGLYARGASPALAGLUGLU
9   VALARGTHRGLUVALLEULYSCYSVALASP
10   LYSASNTHRASPASNASNALACYSHISTRP
11   ALAPHEARGGLYPHELYSCYSPHEGLNLYS
12   ASNASNLEUSERLEUILELYSALASERILE
13   LYSLYSASP

Samples:

H2O: Mol2, [U-95% 13C; U-95% 15N], 0.65 ± 1e-05 mM; sodium phosphate 20 mM

CondSet1: ionic strength: 20.000 mM; pH: 6.500; pressure: 820.000 mbar; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCH2OisotropicCondSet1
3D HNCACBH2OisotropicCondSet1
APO_CBCACONH (H[N[co[{CA|ca[C]}]]])H2OisotropicCondSet1

Software:

CcpNmr_Analysis v2.4, CCPN - Peal Picking

nmrDraw vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum analysis, Spectrum display

nmrPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Varian - Spectrum processing, collection

IstHMS vv211, Hyberts, Milbrandt, Wagner, Haribabu & Wagner - collection, processing

NMR spectrometers:

  • Varian UnityInova 600 MHz

Related Database Links:

UniProt Q1HRL7
AlphaFold Q1HRL7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks