BMRB Entry 27695

Title:
aromatic 1H, 13C chemical shift assignment for FK506 bound FKBP12 C22A
Deposition date:
2018-11-20
Original release date:
2018-11-21
Authors:
Weininger, Ulrich; Akke, Mikael
Citation:

Citation: Weininger, Ulrich; Modig, Kristofer; Geitner, Anne; Schmidpeter, Philipp; Koch, Johanna; Akke, Mikael. "Dynamics of Aromatic Side Chains in the Active Site of FKBP12"  Biochemistry 56, 334-343 (2017).
PubMed: 27936610

Assembly members:

Assembly members:
FKBP12, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet11a

Data sets:
Data typeCount
13C chemical shifts22
1H chemical shifts22

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FKBP121

Entities:

Entity 1, FKBP12 107 residues - Formula weight is not available

1   GLYVALGLNVALGLUTHRILESERPROGLY
2   ASPGLYARGTHRPHEPROLYSARGGLYGLN
3   THRALAVALVALHISTYRTHRGLYMETLEU
4   GLUASPGLYLYSLYSPHEASPSERSERARG
5   ASPARGASNLYSPROPHELYSPHEMETLEU
6   GLYLYSGLNGLUVALILEARGGLYTRPGLU
7   GLUGLYVALALAGLNMETSERVALGLYGLN
8   ARGALALYSLEUTHRILESERPROASPTYR
9   ALATYRGLYALATHRGLYHISPROGLYILE
10   ILEPROPROHISALATHRLEUVALPHEASP
11   VALGLULEULEULYSLEUGLU

Samples:

sample_1: FKBP12, selective 13C, 1 mM; sodium phosphate 25 mM

sample_conditions_1: ionic strength: 25 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian Direct Drive 600 MHz