BMRB Entry 27666

Title:
Resonance assignment of human LARP4A La module
Deposition date:
2018-10-29
Original release date:
2019-02-06
Authors:
Cruz-Gallardo, Isabel; Martino, Luigi; Trotta, Roberta; De Tito, Stefano; Atkinson, Andrew; Conte, Maria
Citation:

Citation: Cruz-Gallardo, Isabel; Martino, Luigi; Trotta, Roberta; De Tito, Stefano; Atkinson, Andrew; Conte, Maria. "Resonance assignment of human LARP4A La module"  Biomol. NMR Assignments 13, 169-172 (2019).
PubMed: 30632004

Assembly members:

Assembly members:
LARP4A, polymer, 179 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-DUET1

Data sets:
Data typeCount
13C chemical shifts543
15N chemical shifts158
1H chemical shifts1035

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LARP4A1

Entities:

Entity 1, LARP4A 179 residues - Formula weight is not available

1   GLNSERASNSERALAVALSERTHRGLUASP
2   LEULYSGLUCYSLEULYSLYSGLNLEUGLU
3   PHECYSPHESERARGGLUASNLEUSERLYS
4   ASPLEUTYRLEUILESERGLNMETASPSER
5   ASPGLNPHEILEPROILETRPTHRVALALA
6   ASNMETGLUGLUILELYSLYSLEUTHRTHR
7   ASPPROASPLEUILELEUGLUVALLEUARG
8   SERSERPROMETVALGLNVALASPGLULYS
9   GLYGLULYSVALARGPROSERHISLYSARG
10   CYSILEVALILELEUARGGLUILEPROGLU
11   THRTHRPROILEGLUGLUVALLYSGLYLEU
12   PHELYSSERGLUASNCYSPROLYSVALILE
13   SERCYSGLUPHEALAHISASNSERASNTRP
14   TYRILETHRPHEGLNSERASPTHRASPALA
15   GLNGLNALAPHELYSTYRLEUARGGLUGLU
16   VALLYSTHRPHEGLNGLYLYSPROILEMET
17   ALAARGILELYSALAILEASNTHRPHEPHE
18   ALALYSASNGLYTYRARGLEUMETASP

Samples:

sample_1: LARP4A, [U-100% 13C; U-100% 15N], 400 uM; LARP4A, [U-100% 15N], 400 uM; TRIS 20 mM; KCL 100 mM; DTT 1 mM; EDTA 0.2 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.25; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment

CCPN, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 950 MHz
  • Bruker EON 800 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks